APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Raji , NCI-H929 , BIRC2-transfected 293T
Predict Reactivity
Mouse, Rat, Dog, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human cIAP1. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888108
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
baculoviral IAP repeat containing 2 , API1 , HIAP2 , Hiap-2 , MIHB , RNF48 , c-IAP1 , cIAP1
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-cIAP1 antibody [GT422] used in Western Blot (WB). GTX629402](/templates/default/js/lightbox/images/loading.gif "Anti-cIAP1 antibody [GT422] used in Western Blot (WB). GTX629402")
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GTX629402 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody [GT422] (GTX629402) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629402 WB Image
cIAP1 antibody [GT422] detects cIAP1 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
7.5 % SDS-PAGE
cIAP1 antibody [GT422] (GTX629402) dilution: 1:250
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GTX629402 ICC/IF Image
cIAP1 antibody [GT422] detects cIAP1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: cIAP1 stained by cIAP1 antibody [GT422] (GTX629402) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar= 10μm.
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GTX629402 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody [GT422] (GTX629402) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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REFERENCE
There are currently no references for cIAP1 antibody [GT422] (GTX629402). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for cIAP1 antibody [GT422] (GTX629402). Be the first to share your experience with this product.
![Anti-cIAP1 antibody [GT422] used in Western Blot (WB). GTX629402](/upload/website/prouct_img/small/GTX629402/GTX629402_41344_20180914_WB_B_w_23061202_916.webp "Anti-cIAP1 antibody [GT422] used in Western Blot (WB). GTX629402")
![Anti-cIAP1 antibody [GT422] used in Western Blot (WB). GTX629402](/upload/website/prouct_img/small/GTX629402/GTX629402_41344_WB_w_23061202_552.webp "Anti-cIAP1 antibody [GT422] used in Western Blot (WB). GTX629402")
![Anti-cIAP1 antibody [GT422] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629402](/upload/website/prouct_img/small/GTX629402/GTX629402_41344_20180321_ICC_IF_w_23061202_980.webp "Anti-cIAP1 antibody [GT422] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629402")
![Anti-cIAP1 antibody [GT422] used in Western Blot (WB). GTX629402](/upload/website/prouct_img/small/GTX629402/GTX629402_41344_20181026_WB_shRNA_watermark_w_23061202_179.webp "Anti-cIAP1 antibody [GT422] used in Western Blot (WB). GTX629402")
![Anti-cIAP1 antibody [GT422] used in Western Blot (WB). GTX629402](/upload/website/prouct_img/normal/GTX629402/GTX629402_41344_20180914_WB_B_w_23061202_916.webp "Anti-cIAP1 antibody [GT422] used in Western Blot (WB). GTX629402")
![Anti-cIAP1 antibody [GT422] used in Western Blot (WB). GTX629402](/upload/website/prouct_img/normal/GTX629402/GTX629402_41344_WB_w_23061202_552.webp "Anti-cIAP1 antibody [GT422] used in Western Blot (WB). GTX629402")
![Anti-cIAP1 antibody [GT422] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629402](/upload/website/prouct_img/normal/GTX629402/GTX629402_41344_20180321_ICC_IF_w_23061202_980.webp "Anti-cIAP1 antibody [GT422] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629402")
![Anti-cIAP1 antibody [GT422] used in Western Blot (WB). GTX629402](/upload/website/prouct_img/normal/GTX629402/GTX629402_41344_20181026_WB_shRNA_watermark_w_23061202_179.webp "Anti-cIAP1 antibody [GT422] used in Western Blot (WB). GTX629402")