APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Dog, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human cIAP1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2909931
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
baculoviral IAP repeat containing 2 , API1 , HIAP2 , Hiap-2 , MIHB , RNF48 , c-IAP1 , cIAP1
Cellular Localization
Cytoplasm,Nucleus
Background
The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Database
Research Area
DATA IMAGES
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GTX635979 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody [HL1045] (GTX635979) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635979 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with cIAP1 antibody [HL1045] (GTX635979) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX635979 IHC-P Image
cIAP1 antibody [HL1045] detects cIAP1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat spleen.cIAP1 stained by cIAP1 antibody [HL1045] (GTX635979) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635979 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody [HL1045] (GTX635979) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635979 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody [HL1045] (GTX635979) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635979 IHC-P Image
cIAP1 antibody [HL1045] detects cIAP1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.cIAP1 stained by cIAP1 antibody [HL1045] (GTX635979) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for cIAP1 antibody [HL1045] (GTX635979). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for cIAP1 antibody [HL1045] (GTX635979). Be the first to share your experience with this product.