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cIAP1 antibody

Anti-cIAP1 antibody used in Western Blot (WB). GTX110087
Anti-cIAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110087
Anti-cIAP1 antibody used in Western Blot (WB). GTX110087
Anti-cIAP1 antibody used in Western Blot (WB). GTX110087
Anti-cIAP1 antibody used in Western Blot (WB). GTX110087
Anti-cIAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110087
Anti-cIAP1 antibody used in Western Blot (WB). GTX110087

Cat. No. GTX110087

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Rat muscle , NCI-H929 , BIRC2-transfected 293T

Predict Reactivity

Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human cIAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036571

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

baculoviral IAP repeat containing 2 , API1 , HIAP2 , Hiap-2 , MIHB , RNF48 , c-IAP1 , cIAP1

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-cIAP1 antibody used in Western Blot (WB). GTX110087

GTX110087 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody (GTX110087) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-cIAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110087

GTX110087 IHC-P Image

cIAP1 antibody detects cIAP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
cIAP1 stained by cIAP1 antibody (GTX110087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-cIAP1 antibody used in Western Blot (WB). GTX110087

GTX110087 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody (GTX110087) diluted at 1:1000 and competitor's antibody (SC-7943) diluted by 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-cIAP1 antibody used in Western Blot (WB). GTX110087

GTX110087 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody (GTX110087) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-cIAP1 antibody used in Western Blot (WB). GTX110087

GTX110087 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody (GTX110087) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-cIAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110087

GTX110087 IHC-P Image

cIAP1 antibody detects cIAP1 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
cIAP1 antibody (GTX110087) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-cIAP1 antibody used in Western Blot (WB). GTX110087

GTX110087 WB Image

Sample (50 μg of whole cell lysate)
A: Rat muscle
7.5% SDS PAGE
GTX110087 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

cIAP1 antibody Cat. No. GTX110087

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 14-May-2012
Score :
Application Tested : WB
Sample Species : Ms
Sample : B cells,T cells,Thymocyte tissue
Amount used : 30μg
Blocking : 0.1% tween-20 in 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169