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cIAP1 antibody

Comparison Orthogonal Overexpression
Anti-cIAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110087
Anti-cIAP1 antibody used in Western Blot (WB). GTX110087
Anti-cIAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110087
Anti-cIAP1 antibody used in Western Blot (WB). GTX110087
Anti-cIAP1 antibody used in Western Blot (WB). GTX110087
Anti-cIAP1 antibody used in Western Blot (WB). GTX110087

Cat. No. GTX110087

Reference 8
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all cIAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Rat muscle , NCI-H929 , BIRC2-transfected 293T

Predict Reactivity

Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human cIAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036571

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

baculoviral IAP repeat containing 2 , API1 , HIAP2 , Hiap-2 , MIHB , RNF48 , c-IAP1 , cIAP1

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-cIAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110087

GTX110087 IHC-P Image

cIAP1 antibody detects cIAP1 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
cIAP1 antibody (GTX110087) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-cIAP1 antibody used in Western Blot (WB). GTX110087

GTX110087 WB Image

Sample (50 μg of whole cell lysate)
A: Rat muscle
7.5% SDS PAGE
GTX110087 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-cIAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110087

GTX110087 IHC-P Image

cIAP1 antibody detects cIAP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
cIAP1 stained by cIAP1 antibody (GTX110087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-cIAP1 antibody used in Western Blot (WB). GTX110087

GTX110087 WB Image

Comparison

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody (GTX110087) diluted at 1:1000 and competitor's antibody (SC-7943) diluted by 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-cIAP1 antibody used in Western Blot (WB). GTX110087

GTX110087 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody (GTX110087) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-cIAP1 antibody used in Western Blot (WB). GTX110087

GTX110087 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody (GTX110087) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Liu F et al. Adv Sci (Weinh) 2022; 9 (22) : e2104823 A Temporal PROTAC Cocktail-Mediated Sequential Degradation of AURKA Abrogates Acute Myeloid Leukemia Stem Cells.
Application : WB
Reactivity : Human
Lin CY et al. Int J Mol Sci. 2021; 22 (20) : 11020 Inhibition of BIRC2 Sensitizes α7-HPV-Related Cervical Squamous Cell Carcinoma to Chemotherapy.
Application : WB
Reactivity : Human
Wu YH et al. Cell Death Dis 2020; 11 (5) : 305 Tumor suppressor death-associated protein kinase 1 inhibits necroptosis by p38 MAPK activation.
Application : WB
Reactivity : Human
Xu YM et al. Oncotarget 2016; 7 (5) : 6146-58 Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
Reactivity : Human
Leu JS et al. Am J Respir Crit Care Med 2016; SP110b Controls Host Immunity and Susceptibility to Tuberculosis.
Application : WB
Reactivity : Human
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REVIEW

cIAP1 antibody Cat. No. GTX110087

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-cIAP1 antibody used in Western Blot (WB). GTX110087
Date : Anonymous submitted on 14-May-2012
Score :
Application Tested : WB
Sample Species : Ms
Sample : B cells,T cells,Thymocyte tissue
Amount used : 30μg
Blocking : 0.1% tween-20 in 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169