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cK-Ras antibody [234-4.2]

Cat No. GTX82497

Host Mouse
Clonality Monoclonal
Clone Name 234-4.2
Isotype IgG2a
Application WB, IHC-P, IHC-Fr, IP
Reactivity Human, Mouse, Rat
Package
50μg ($329)
APPLICATION

Application Note

For IHC-Fr: Use at a concentration of 10 μg/ml. For IHC-P: Use at a concentration of 10 μg/ml. For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 5 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

22 kDa. ( Note )

Positive Control

SW480, Y1 cells, or normal skin tissue

Specificity/Sensitivity

This antibody reacts with cK Ras and vK Ras, and cross-reacts with cH Ras or cN Ras p21s in immunoblotting. For this reason we recommend a sample concentration step prior to immunoblotting; concentrate to 5 μg/ml with 1% gelatin as a stabilizer. For immunoprecipitation, use 5 μg per sample with 45 μl protein A agarose.
PROPERTIES

Form

Liquid

Buffer

50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5, containing 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Recombinant fragment, corresponding to C terminal amino acids 54-189 of Rat cK RAS.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Kras Proto-Oncogene, Gtpase,C-K-Ras,Cfc2,K-Ras2A,K-Ras2B,K-Ras4A,K-Ras4B,K-Ras,Ki-Ras,Kras1,Kras2,Ns,Ns3,Rald,Rask2,C-Ki-Ras2,Kras

Background

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE
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REVIEW
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Package List Price ($)
$ 329