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caspILLUME Green Active Caspase-3 Staining Kit

caspILLUME Green Active Caspase-3 Staining Kit. GTX85555
caspILLUME Green Active Caspase-3 Staining Kit. GTX85555

Cat No. GTX85555

Application FACS
Package
1 kit ($399)
PRODUCT

Product Description

Activation of caspases plays a central role in apoptosis. The CaspILLUME? Fluorescein Active Caspase-3 Staining Kit provides a convenient means for sensitive detection of activated caspase-3 in living cells. The assay utilizes the caspase-3 inhibitor, DEVD-FMK, conjugated to FITC (FITC-DEVD-FMK) as a marker. FITC-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells.

‧ Detection method- Fluorescence microscopy, Flow cytometry and plate reader (Ex. = 485 nm and Em. = 535 nm)
‧ Sample type- Live cells
‧ Species reactivity- Mammalian
‧ Applications- Sensitive detection of activated caspase-3 in living cells.

Features and Benefits
‧ Simple one-step procedure; takes only 1-2 hours
‧ Fast and convenient
‧ The FITC label allows for direct detection of the activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.

Kit Contents:
FITC-DEVD-FMK
Wash Buffer
Z-VAD-FMK

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
FACS Assay dependent
Not tested in other applications.
PROPERTIES

Storage

Store at -20ºC. Product has an expected shelf life of 6-12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase-3 Assay, Caspase 3 Activity Assay, Apoptosis Assay, Caspase-3 Activity Assay, Caspase3 Assay, Caspase3 Activity Assay, Caspase 3 Assay
DATA IMAGES
caspILLUME Green Active Caspase-3 Staining Kit. GTX85555

GTX85555 Image

10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.

caspILLUME Green Active Caspase-3 Staining Kit. GTX85555

GTX85555 FACS Image

Detection of Caspase­3 Activation Using CaspILLUME Fluorescein Caspase­3 Staining Kit. Apoptosis was induced in Jurkat cells with (B) or without (A) camptothecin for 6 hours. Cells were collected and incubated with CaspILLUME in situ marker, FITC­DEVD­FMK, for 20 minutes according to kit instructions. Cells were then analyzed by flow cytometry in the FL1 channel.

REFERENCE
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Package List Price ($)
$ 399