‧ Detection method- Fluorescence microscopy, Flow cytometry and plate reader (Ex. = 485 nm and Em. = 535 nm)
‧ Sample type- Live cells
‧ Species reactivity- Mammalian
‧ Applications- Sensitive detection of activated caspase-3 in living cells.
Features and Benefits
‧ Simple one-step procedure; takes only 1-2 hours
‧ Fast and convenient
‧ The FITC label allows for direct detection of the activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
GTX85555 FACS Image
Detection of Caspase3 Activation Using CaspILLUME Fluorescein Caspase3 Staining Kit. Apoptosis was induced in Jurkat cells with (B) or without (A) camptothecin for 6 hours. Cells were collected and incubated with CaspILLUME in situ marker, FITCDEVDFMK, for 20 minutes according to kit instructions. Cells were then analyzed by flow cytometry in the FL1 channel.
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