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caspILLUME Red Active Caspase-9 Staining Kit

caspILLUME Red Active Caspase-9 Staining Kit. GTX85534
caspILLUME Red Active Caspase-9 Staining Kit. GTX85534

Cat No. GTX85534

Application

FACS
Package
100 assay ($529)
PRODUCT

Summary

Activation of caspases plays a central role in apoptosis. The CaspILLUMETM Red Active Caspase-9 Staining Kit provides a convenient means for detecting activated caspase-9 in living cells. The assay utilizes a caspase-9 inhibitor LEHD-FMK conjugated to sulfo-rhodamine (Red-LEHD-FMK) as the fluorescent marker. Red-LEHD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-9 in apoptotic cells.

‧ Detection method- Fluorescence microscopy, plate reader (Ex/Em = 540/570 nm) and Flow cytometry
‧ Sample type- Live cells
‧ Species reactivity- Mammalian
‧ Applications- Detection of activated caspase-9 in living cells.

Features and Benefits
‧ Simple one-step procedure; takes only 1-2 hours
‧ Fast and convenient
‧ The fluorescence label allows detection of activated caspase-9 in apoptotic cells directly by fluorescence microscopy, flow cytometry, or fluorescence plate reader.

Kit Contents:
Red-LEHD-FMK
Wash Buffer
Z-VAD-FMK

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
FACS Assay dependent
Not tested in other applications.

PROPERTIES

Storage

Store at -20ºC. Product has an expected shelf life of 6-12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Caspase 9 Activity Assay , Apoptosis Assay , Caspase-9 Activity Assay , Caspase 9 Assay , Caspase-9 Assay , Caspase9 Assay , Caspase9 Activity Assay

DATA IMAGES

caspILLUME Red Active Caspase-9 Staining Kit. GTX85534

GTX85534 Image

caspILLUME Red Active Caspase-9 Staining Kit. GTX85534

GTX85534 FACS Image

Detection of Caspase­3 Activation Using CaspILLUME Fluorescein Caspase­3 Staining Kit. Apoptosis was induced in Jurkat cells with (B) or without (A) camptothecin for 6 hours. Cells were collected and incubated with CaspILLUME in situ marker, FITC­DEVD­FMK, for 20 minutes according to kit instructions. Cells were then analyzed by flow cytometry in the FL1 channel.

REFERENCE

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Package List Price ($)
$ 529