100 assay ($529)
‧ Detection method- Fluorescence microscopy, plate reader (Ex/Em = 540/570 nm) and Flow cytometry
‧ Sample type- Live cells
‧ Species reactivity- Mammalian
‧ Applications- Detection of activated caspase-9 in living cells.
Features and Benefits
‧ Simple one-step procedure; takes only 1-2 hours
‧ Fast and convenient
‧ The fluorescence label allows detection of activated caspase-9 in apoptotic cells directly by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
GTX85534 FACS Image
Detection of Caspase3 Activation Using CaspILLUME Fluorescein Caspase3 Staining Kit. Apoptosis was induced in Jurkat cells with (B) or without (A) camptothecin for 6 hours. Cells were collected and incubated with CaspILLUME in situ marker, FITCDEVDFMK, for 20 minutes according to kit instructions. Cells were then analyzed by flow cytometry in the FL1 channel.
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