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eEF1A1 antibody [AT23C11]

Anti-eEF1A1 antibody [AT23C11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57614
Anti-eEF1A1 antibody [AT23C11] used in Western Blot (WB). GTX57614

Cat. No. GTX57614

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT23C11

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all eEF1A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT23C11 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human EEF1A3 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation elongation factor 1 alpha 1 , CCS3 , EE1A1 , EEF1 , EEF1A , EEF1A1 , EF1A , EFTu , GRAF1EF , HNGC:16303 , LENG7 , PTI1 , eEF1A1

Cellular Localization

Cytoplasm,Nucleus,Nucleolus,Cell membrane

Background

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-eEF1A1 antibody [AT23C11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57614

GTX57614 ICC/IF Image

ICC/IF analysis of A549 cells using GTX57614 eEF1A1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-eEF1A1 antibody [AT23C11] used in Western Blot (WB). GTX57614

GTX57614 WB Image

WB analysis of various samples using GTX57614 eEF1A1 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : A549 whole cell lysate
Lane 3 : Raji whole cell lysate
Lane 4 : THP-1 whole cell lysate
Lane 5 : MCF-7 whole cell lysate
Loading : 40 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399