GeneTex
  • Country / Location Selection

United States (US)

eEF1A1 antibody [JB44-13]

Recombinant antibody
Anti-eEF1A1 antibody [JB44-13] used in Western Blot (WB). GTX01048
Anti-eEF1A1 antibody [JB44-13] used in IHC (Paraffin sections) (IHC-P). GTX01048
Anti-eEF1A1 antibody [JB44-13] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01048
Anti-eEF1A1 antibody [JB44-13] used in IHC (Paraffin sections) (IHC-P). GTX01048
Anti-eEF1A1 antibody [JB44-13] used in IHC (Paraffin sections) (IHC-P). GTX01048
Anti-eEF1A1 antibody [JB44-13] used in IHC (Paraffin sections) (IHC-P). GTX01048
Anti-eEF1A1 antibody [JB44-13] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01048
Anti-eEF1A1 antibody [JB44-13] used in IHC (Paraffin sections) (IHC-P). GTX01048
Anti-eEF1A1 antibody [JB44-13] used in Flow cytometry (FACS). GTX01048
Anti-eEF1A1 antibody [JB44-13] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01048

Cat No. GTX01048

Host

Rabbit

Clonality

Monoclonal

Clone Name

JB44-13

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse, Rat
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation elongation factor 1 alpha 1,CCS3,EE1A1,EEF1,EEF1A,EEF1A1,EF1A,EFTu,GRAF1EF,HNGC:16303,LENG7,PTI1,eEF1A1

Cellular Localization

Cytoplasm,Nucleus,Nucleolus,Cell membrane

Background

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-eEF1A1 antibody [JB44-13] used in Western Blot (WB). GTX01048

GTX01048 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with eEF1A1 antibody [JB44-13] (GTX01048) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-eEF1A1 antibody [JB44-13] used in IHC (Paraffin sections) (IHC-P). GTX01048

GTX01048 IHC-P Image

IHC-P analysis of mouse smooth muscle tissue section using GTX01048 eEF1A1 antibody [JB44-13].

Anti-eEF1A1 antibody [JB44-13] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01048

GTX01048 ICC/IF Image

ICC/IF analysis of PFA-fixed SH-SY-5Y cells using GTX01048 eEF1A1 antibody [JB44-13].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-eEF1A1 antibody [JB44-13] used in IHC (Paraffin sections) (IHC-P). GTX01048

GTX01048 IHC-P Image

IHC-P analysis of human liver cancer tissue section using GTX01048 eEF1A1 antibody [JB44-13].

Anti-eEF1A1 antibody [JB44-13] used in IHC (Paraffin sections) (IHC-P). GTX01048

GTX01048 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX01048 eEF1A1 antibody [JB44-13].

Anti-eEF1A1 antibody [JB44-13] used in IHC (Paraffin sections) (IHC-P). GTX01048

GTX01048 IHC-P Image

IHC-P analysis of rat skeletal muscle tissue section using GTX01048 eEF1A1 antibody [JB44-13].

Anti-eEF1A1 antibody [JB44-13] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01048

GTX01048 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells using GTX01048 eEF1A1 antibody [JB44-13].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-eEF1A1 antibody [JB44-13] used in IHC (Paraffin sections) (IHC-P). GTX01048

GTX01048 IHC-P Image

IHC-P analysis of human kidney tissue section using GTX01048 eEF1A1 antibody [JB44-13].

Anti-eEF1A1 antibody [JB44-13] used in Flow cytometry (FACS). GTX01048

GTX01048 FACS Image

FACS analysis of THP-1 cells using GTX01048 eEF1A1 antibody [JB44-13].
Red : primary antibody
Black : unlabelled control
Dilution : 1:100

Anti-eEF1A1 antibody [JB44-13] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01048

GTX01048 ICC/IF Image

ICC/IF analysis of PFA-fixed HUVEC cells using GTX01048 eEF1A1 antibody [JB44-13].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

REFERENCE

There are currently no references for eEF1A1 antibody [JB44-13] (GTX01048). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for eEF1A1 antibody [JB44-13] (GTX01048). Be the first to share your experience with this product.