GeneTex
United States (US)

eEF1A1 antibody [N1C1]

GTX102285_WB_19011506_164.jpg

Cat No. GTX102285

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T

Specificity/Sensitivity

Based on a BLAST result, it may also cross with human eEF1A2.

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Cat, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human eEF1A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Elongation Factor 1 Alpha 1,Ccs-3,Ccs3,Ee1A1,Eef-1,Eef1A,Ef-Tu,Ef1A,Graf-1Ef,Hngc:16303,Leng7,Pti1,Eef1A-1,Eef1A1

Cellular Localization

Cytoplasm

Background

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102285_WB_19011506_164.jpg

GTX102285 WB Image

Sample(30 μg of whole cell lysate)
A:293T
7.5% SDS PAGE
GTX102285 diluted at 1:1000

REFERENCE
REVIEW

eEF1A1 antibody [N1C1] Cat No. GTX102285

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 08-Jan-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : PANC1 cells
Amount used : 25 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf