APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Not tested in other applications.
Calculated MW
Product Note
Based on a BLAST result, it may also cross with human eEF1A2.
Predict Reactivity
Rabbit, Bovine, Cat, Dog, Chicken, Pig, Japanese Medaka, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.59 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human eEF1A1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950164
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
eukaryotic translation elongation factor 1 alpha 1 , CCS-3 , CCS3 , EE1A1 , EEF-1 , EEF1A , EF-Tu , EF1A , GRAF-1EF , HNGC:16303 , LENG7 , PTI1 , eEF1A-1
Cellular Localization
Cytoplasm
Background
This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX102285 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with eEF1A1 antibody [N1C1] (GTX102285) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX102285 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with eEF1A1 antibody [N1C1] (GTX102285) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX102285 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with eEF1A1 antibody [N1C1] (GTX102285) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX102285 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with eEF1A1 antibody [N1C1] (GTX102285) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
eEF1A1 antibody [N1C1]
Cat. No. GTX102285
Rating
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( Average 5 based on 1 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 08-Jan-2018
Application Tested :
WB
Sample Species :
Hu
Sample :
PANC1 cells
Amount used :
25 μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:500, 4°C, 16Hr