eEF1A1 antibody [N1C1]

Name: eEF1A1 antibody [N1C1]
Brand: GeneTex
SKU: GTX102285
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-eEF1A1 antibody [N1C1] used in Western Blot (WB). GTX102285

Cat. No. GTX102285

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human, Mouse, Rat, Golden Syrian Hamster
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX102285 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Product Note

Based on a BLAST result, it may also cross with human eEF1A2.

Predict Reactivity

Rabbit, Bovine, Cat, Dog, Chicken, Pig, Japanese Medaka, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human eEF1A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950164

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation elongation factor 1 alpha 1 , CCS-3 , CCS3 , EE1A1 , EEF-1 , EEF1A , EF-Tu , EF1A , GRAF-1EF , HNGC:16303 , LENG7 , PTI1 , eEF1A-1

Cellular Localization

Cytoplasm

Background

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq]

Database

Gene ID: 1915 EEF1A1
UniProt: P68104 EEF1A1

Research Area

DATA IMAGES

GTX102285 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with eEF1A1 antibody [N1C1] (GTX102285) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102285 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with eEF1A1 antibody [N1C1] (GTX102285) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102285 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with eEF1A1 antibody [N1C1] (GTX102285) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102285 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with eEF1A1 antibody [N1C1] (GTX102285) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Shen TJ et al. JCI Insight 2022; 7 (21) Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein-mediated viral translation.

Application : WB

Reactivity : Golden Syrian Hamster

Ko CC et al. J Biol Chem 2014; Chemical proteomics identifies heterogeneous nuclear ribonucleoprotein (hnRNP) A1 as the molecular target of Quercetin in its anti-cancer effects in PC-3 cells.

Application : WB

Reactivity : Human

Cheng YC et al. Mol Neurobiol 2012; 45 (3) : 536-49 3'poly-G-tailed ODNs inhibit F-spondin to induce cell death and neurite retraction in rat embryonic neurons.

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