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eEF1A1 antibody

Anti-eEF1A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02242
Anti-eEF1A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02242
Anti-eEF1A1 antibody used in Western Blot (WB). GTX02242
Anti-eEF1A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02242

Cat No. GTX02242

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:300-1:10000
IHC-P 1:200-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH7.0)

Preservative

0.03% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to the center region of the Human EEF1A1

Purification

Purified by Protein A and Antigen Affinity

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation elongation factor 1 alpha 1 , CCS-3 , CCS3 , EE1A1 , EEF-1 , EEF1A , EF-Tu , EF1A , GRAF-1EF , HNGC:16303 , LENG7 , PTI1 , eEF1A-1

Cellular Localization

Cytoplasm , Nucleus , Cell membrane , Nucleolus

Background

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-eEF1A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02242

GTX02242 IHC-P Image

IHC-P analysis of human breast carcinoma tissue section using GTX02242 eEF1A1 antibody.
Dilution : 1:500

Anti-eEF1A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02242

GTX02242 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX02242 eEF1A1 antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 1:1000
Permeabilzation : 0.1% Triton X-100

Anti-eEF1A1 antibody used in Western Blot (WB). GTX02242

GTX02242 WB Image

WB analysis of various samples using GTX02242 eEF1A1 antibody.
Lane A : HeLa whole cell lysate
Lane B : MCF-7 whole cell lysate
Lane C : HEK293 whole cell lysate
Dilution : 1:500
Loading : 30 μg

Anti-eEF1A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02242

GTX02242 IHC-P Image

IHC-P analysis of human brain tissue section using GTX02242 eEF1A1 antibody.
Dilution : 1:500

REFERENCE

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REVIEW

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Package List Price ($)
$ 339