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eEF1A1 antibody

Anti-eEF1A1 antibody used in Western Blot (WB). GTX54012
Anti-eEF1A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54012
Anti-eEF1A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54012
Anti-eEF1A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54012

Cat. No. GTX54012

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all eEF1A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:100
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Product Note

Based on a BLAST result, it may also cross with human eEF1A2.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 123-462 of human EEF1A1 (NP_001393.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation elongation factor 1 alpha 1 , CCS-3 , CCS3 , EE1A1 , EEF-1 , EEF1A , EF-Tu , EF1A , GRAF-1EF , HNGC:16303 , LENG7 , PTI1 , eEF1A-1

Cellular Localization

Cytoplasm,Nucleus,Cell membrane,Nucleolus

Background

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-eEF1A1 antibody used in Western Blot (WB). GTX54012

GTX54012 WB Image

WB analysis of various sample lysates using GTX54012 eEF1A1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-eEF1A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54012

GTX54012 ICC/IF Image

ICC/IF analysis of C6 cells using GTX54012 eEF1A1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-eEF1A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54012

GTX54012 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54012 eEF1A1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-eEF1A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54012

GTX54012 ICC/IF Image

ICC/IF analysis of L929 cells using GTX54012 eEF1A1 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399