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eEF2 antibody [5B6]

Anti-eEF2 antibody [5B6] used in Western Blot (WB). GTX60421
Anti-eEF2 antibody [5B6] used in ELISA (ELISA). GTX60421
Anti-eEF2 antibody [5B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60421
Anti-eEF2 antibody [5B6] used in IHC (Paraffin sections) (IHC-P). GTX60421
Anti-eEF2 antibody [5B6] used in IHC (Paraffin sections) (IHC-P). GTX60421

Cat. No. GTX60421

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5B6

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all eEF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

95 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human EEF2 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation elongation factor 2 , EEF-2 , EF-2 , EF2 , SCA26

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Anti-eEF2 antibody [5B6] used in Western Blot (WB). GTX60421

GTX60421 WB Image

WB analysis of HepG2 (1), HeLa (2), HEK293 (3) and A431 (4) cell lysate using GTX60421 eEF2 antibody [5B6].

Anti-eEF2 antibody [5B6] used in ELISA (ELISA). GTX60421

GTX60421 ELISA Image

ELISA analysis of antigen using GTX60421 eEF2 antibody [5B6].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-eEF2 antibody [5B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60421

GTX60421 ICC/IF Image

ICC/IF analysis of 3T3-L1 cells using GTX60421 eEF2 antibody [5B6].
Green : eEF2
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-eEF2 antibody [5B6] used in IHC (Paraffin sections) (IHC-P). GTX60421

GTX60421 IHC-P Image

IHC-P analysis of liver cancer tissue (left) and kidney cancer tissue (right) using GTX60421 eEF2 antibody [5B6].

Anti-eEF2 antibody [5B6] used in IHC (Paraffin sections) (IHC-P). GTX60421

GTX60421 IHC-P Image

IHC-P analysis of prostate cancer tissue (left) and tonsil tissue (right) using GTX60421 eEF2 antibody [5B6].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399