APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , GL261 , NIH-3T3 , C2C12 , Neuro 2A , Raw264.7 , PC-12
Predict Reactivity
Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.86 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human eEF2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950169
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
eukaryotic translation elongation factor 2 , EEF-2 , EF-2 , EF2 , SCA26
Cellular Localization
Cytoplasm
Background
This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq]
Database
DATA IMAGES
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GTX102287 IHC-P Image
eEF2 antibody detects eEF2 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. eEF2 antibody (GTX102287) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX102287 IHC-P Image
eEF2 antibody detects eEF2 protein at cytosol on human colon by immunohistochemical analysis. Sample: Paraffin-embedded colon. eEF2 antibody (GTX102287) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX102287 WB Image
Sample(30 μg of whole cell lysate) A:293T B:A431(GTX27909) 7.5% SDS PAGE GTX102287 diluted at 1:1500
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GTX102287 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal (GTX102287) diluted at 1:500.
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GTX102287 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal (GTX102287) diluted at 1:500.
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GTX102287 IHC-P Image
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using eEF2 antibody [N2C1], Internal (GTX102287) at 1:300 dilution.
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GTX102287 WB Image
Sample (30 μg of whole cell lysate) A: zebrafish eye 7.5% SDS PAGE GTX102287 diluted at 1:1000
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GTX102287 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal (GTX102287) diluted at 1:1000.
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GTX102287 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal (GTX102287) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Drosophila eEF2 (Gene ID: 35422/ UniProt: P13060)
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REFERENCE
There are currently no references for eEF2 antibody [N2C1], Internal (GTX102287). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for eEF2 antibody [N2C1], Internal (GTX102287). Be the first to share your experience with this product.