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eEF2 antibody [N2C1], Internal

Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287
Anti-eEF2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102287
Anti-eEF2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102287
Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287
Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287
Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287
Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287
Anti-eEF2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102287
Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287

Cat. No. GTX102287

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all eEF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

95 kDa. ( Note )

Positive Control

293T , A431 , GL261 , NIH-3T3 , C2C12 , Neuro 2A , Raw264.7 , PC-12

Predict Reactivity

Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human eEF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950169

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation elongation factor 2 , EEF-2 , EF-2 , EF2 , SCA26

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq]

Database

DATA IMAGES

Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287

GTX102287 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal (GTX102287) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Drosophila eEF2 (Gene ID: 35422/ UniProt: P13060)

Anti-eEF2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102287

GTX102287 IHC-P Image

eEF2 antibody detects eEF2 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
eEF2 antibody (GTX102287) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-eEF2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102287

GTX102287 IHC-P Image

eEF2 antibody detects eEF2 protein at cytosol on human colon by immunohistochemical analysis.
Sample: Paraffin-embedded colon.
eEF2 antibody (GTX102287) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287

GTX102287 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
7.5% SDS PAGE
GTX102287 diluted at 1:1500

Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287

GTX102287 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal (GTX102287) diluted at 1:500.

Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287

GTX102287 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal (GTX102287) diluted at 1:500.

Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287

GTX102287 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal (GTX102287) diluted at 1:1000.

Anti-eEF2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102287

GTX102287 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using eEF2 antibody [N2C1], Internal (GTX102287) at 1:300 dilution.

Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287

GTX102287 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
7.5% SDS PAGE
GTX102287 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169