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eEF2K antibody [N1N3]

Anti-eEF2K antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111496
Anti-eEF2K antibody [N1N3] used in Western Blot (WB). GTX111496
Anti-eEF2K antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111496
Anti-eEF2K antibody [N1N3] used in Western Blot (WB). GTX111496
Anti-eEF2K antibody [N1N3] used in Western Blot (WB). GTX111496

Cat. No. GTX111496

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all eEF2K products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

Mouse liver , A431 , H1299 , HeLa , HepG2 , PC-12

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human eEF2K. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950170

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic elongation factor 2 kinase , CaMKIII , HSU93850 , eEF-2K

Background

This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-eEF2K antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111496

GTX111496 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using eEF-2K(GTX111496) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-eEF2K antibody [N1N3] used in Western Blot (WB). GTX111496

GTX111496 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2K antibody [N1N3] (GTX111496) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-eEF2K antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111496

GTX111496 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using eEF-2K(GTX111496) antibody at 1:200 dilution.

Anti-eEF2K antibody [N1N3] used in Western Blot (WB). GTX111496

GTX111496 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX111496 diluted at 1:1000

Anti-eEF2K antibody [N1N3] used in Western Blot (WB). GTX111496

GTX111496 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: Hela
D: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX111496 diluted at 1:5000

REFERENCE

Application Reference

XL Wang et al. Research Square 2024; Reduction of eEF2 kinase alleviates the learning and memory impairment caused by acrylamide
Liong S et al. Reprod Fertil Dev 2017; (Epub) Markers of protein synthesis are increased in fetal membranes and myometrium after human labour and delivery.
Application : WB
Reactivity : Human
Usui T et al. Acta Physiol (Oxf) 2014; Eukaryotic elongation factor2 kinase controls proliferation and migration of vascular smooth muscle cells.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169