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eEF2K antibody

KO/KD
Anti-eEF2K antibody used in Western Blot (WB). GTX32573
Anti-eEF2K antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32573
Anti-eEF2K antibody used in Western Blot (WB). GTX32573

Cat. No. GTX32573

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($409)
See all eEF2K products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 406-725 of human EEF2K (NP_037434.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic elongation factor 2 kinase , CaMKIII , HSU93850 , eEF-2K

Background

This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-eEF2K antibody used in Western Blot (WB). GTX32573

GTX32573 WB Image

WB analysis of various sample lysates using GTX32573 eEF2K antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-eEF2K antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32573

GTX32573 ICC/IF Image

ICC/IF analysis of A549 cells using GTX32573 eEF2K antibody.
Blue : DAPI

Anti-eEF2K antibody used in Western Blot (WB). GTX32573

GTX32573 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX32573 eEF2K antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409