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eEF2K (phospho Ser366) antibody

Anti-eEF2K (phospho Ser366) antibody used in Western Blot (WB). GTX32365
Anti-eEF2K (phospho Ser366) antibody used in IHC (Paraffin sections) (IHC-P). GTX32365

Cat. No. GTX32365

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all eEF2K products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of eEF-2K. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic elongation factor 2 kinase , CaMKIII , HSU93850 , eEF-2K

Background

This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-eEF2K (phospho Ser366) antibody used in Western Blot (WB). GTX32365

GTX32365 WB Image

WB analysis of Calyculin A-treated HeLa (A) whole cell lysates using GTX32365 eEF-2K (phospho Ser366) antibody.

Anti-eEF2K (phospho Ser366) antibody used in IHC (Paraffin sections) (IHC-P). GTX32365

GTX32365 IHC-P Image

IHC-P analysis of formalin fixed human prostate cancer tissue section using GTX32365 eEF-2K (phospho Ser366) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399