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eEF2K (phospho Ser366) antibody

Anti-eEF2K (phospho Ser366) antibody used in Western Blot (WB). GTX32365
Anti-eEF2K (phospho Ser366) antibody used in IHC (Paraffin sections) (IHC-P). GTX32365

Cat No. GTX32365

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of eEF-2K. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic elongation factor 2 kinase , CaMKIII , HSU93850 , eEF-2K

Background

This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-eEF2K (phospho Ser366) antibody used in Western Blot (WB). GTX32365

GTX32365 WB Image

WB analysis of Calyculin A-treated HeLa (A) whole cell lysates using GTX32365 eEF-2K (phospho Ser366) antibody.

Anti-eEF2K (phospho Ser366) antibody used in IHC (Paraffin sections) (IHC-P). GTX32365

GTX32365 IHC-P Image

IHC-P analysis of formalin fixed human prostate cancer tissue section using GTX32365 eEF-2K (phospho Ser366) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

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REVIEW

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Package List Price ($)
$ 319