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eIF2 alpha antibody [1H1]

Overexpression
Anti-eIF2 alpha antibody [1H1] used in IHC (Paraffin sections) (IHC-P). GTX84582
Anti-eIF2 alpha antibody [1H1] used in Western Blot (WB). GTX84582
Anti-eIF2 alpha antibody [1H1] used in Western Blot (WB). GTX84582
Anti-eIF2 alpha antibody [1H1] used in Flow cytometry (FACS). GTX84582

Cat. No. GTX84582

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1H1

Isotype

 IgG1

Application

 WB, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Dog, Monkey
Package
100 μl ($519)
See all eIF2 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
IHC-P 1:150
FACS 1:100
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human EIF2S1 (NP_004085) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2 subunit alpha , EIF-2 , EIF-2A , EIF-2alpha , EIF2 , EIF2A

Background

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Database

Research Area

DATA IMAGES

Anti-eIF2 alpha antibody [1H1] used in IHC (Paraffin sections) (IHC-P). GTX84582

GTX84582 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84582 eIF2 alpha antibody [1H1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-eIF2 alpha antibody [1H1] used in Western Blot (WB). GTX84582

GTX84582 WB Image

Overexpression

WB analysis of HEK293T cells transfected with eIF2 alpha plasmid (Right) or empty vector (Left) for 48 hrs using GTX84582 eIF2 alpha antibody [1H1].
Loading : 5 ug per lane

Anti-eIF2 alpha antibody [1H1] used in Western Blot (WB). GTX84582

GTX84582 WB Image

WB analysis of various cell lines using GTX84582 eIF2 alpha antibody [1H1].
Loading : 35 ug per lane
Dilution : 1:200

Anti-eIF2 alpha antibody [1H1] used in Flow cytometry (FACS). GTX84582

GTX84582 FACS Image

FACS analysis of Jurkat cells using GTX84582 eIF2 alpha antibody [1H1].
Red : Primary antibody
Blue : Negative control antibody

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519