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eIF2 alpha antibody [2E4]

Anti-eIF2 alpha antibody [2E4] used in Western Blot (WB). GTX66838
Anti-eIF2 alpha antibody [2E4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66838

Cat No. GTX66838

Host

Mouse

Clonality

Monoclonal

Clone Name

2E4

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:50-1:200
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine pH 7.4, 150 mM NaCl, 0.2% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human eIF2α protein fragments expressed in E.coli.

Purification

Purified by antigen-affinity chromatography

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation initiation factor 2 subunit alpha , EIF-2 , EIF-2A , EIF-2alpha , EIF2 , EIF2A

Background

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-eIF2 alpha antibody [2E4] used in Western Blot (WB). GTX66838

GTX66838 WB Image

WB analysis of K562, COS7, 3T3, C2C12, MCF-7, C6, and HeLa cell lysates using GTX66838 eIF2 alpha antibody [2E4].
Dilution : 1:1000

Anti-eIF2 alpha antibody [2E4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66838

GTX66838 ICC/IF Image

ICC/IF analysis of 4% PFA-fixed HeLa cells using GTX66838 eIF2 alpha antibody [2E4].
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf