eIF2 alpha antibody [3H4]

Cat. No. GTX84579

Host	Mouse
Clonality	Monoclonal
Clone Name	3H4
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human, Rat, Dog, Monkey
Package	100 μl ($519)

See all eIF2 alpha products

Datasheet
Document

View product citations for antibody GTX84579 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:2000
ICC/IF	1:100
IHC-P	1:50
FACS	1:100

Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human EIF2S1 (NP_004085) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2 subunit alpha , EIF-2 , EIF-2A , EIF-2alpha , EIF2 , EIF2A

Background

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Database

Gene ID: 1965 EIF2S1
UniProt: P05198 EIF2S1

Research Area

DATA IMAGES

GTX84579 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84579 eIF2 alpha antibody [3H4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84579 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84579 eIF2 alpha antibody [3H4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84579 FACS Image

FACS analysis of Jurkat cells using GTX84579 eIF2 alpha antibody [3H4].
Red : Primary antibody
Blue : Negative control antibody

GTX84579 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX84579 eIF2 alpha antibody [3H4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84579 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with eIF2 alpha plasmid using GTX84579 eIF2 alpha antibody [3H4].

GTX84579 FACS Image

FACS analysis of HEK293T cells transfected with either eIF2 alpha plasmid(Red) or empty vector control plasmid(Blue) using GTX84579 eIF2 alpha antibody [3H4].

GTX84579 WB Image

WB analysis of various cell lines using GTX84579 eIF2 alpha antibody [3H4].
Loading : 35 ug per lane

GTX84579 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84579 eIF2 alpha antibody [3H4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84579 WB Image

WB analysis of HEK293T cells transfected with eIF2 alpha plasmid (Right) or empty vector (Left) for 48 hrs using GTX84579 eIF2 alpha antibody [3H4].
Loading : 5 ug per lane

GTX84579 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84579 eIF2 alpha antibody [3H4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

eIF2 alpha antibody [3H4]

Cat. No. GTX84579

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Background

Database

Research Area

DATA IMAGES

GTX84579 IHC-P Image

GTX84579 IHC-P Image

GTX84579 FACS Image

GTX84579 IHC-P Image

GTX84579 ICC/IF Image

GTX84579 FACS Image

GTX84579 WB Image

GTX84579 IHC-P Image

GTX84579 WB Image

GTX84579 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.