GeneTex

  • Country / Location Selection

United States (US)

eIF2 alpha antibody [EIF2a]

Anti-eIF2 alpha antibody [EIF2a] used in IHC (Paraffin sections) (IHC-P). GTX25369
Anti-eIF2 alpha antibody [EIF2a] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25369
Anti-eIF2 alpha antibody [EIF2a] used in IHC (Paraffin sections) (IHC-P). GTX25369
Anti-eIF2 alpha antibody [EIF2a] used in Western Blot (WB). GTX25369

Cat. No. GTX25369

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 EIF2a

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Primate
Package
50 μg ($399)
See all eIF2 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:250
IHC-P 1:10-1:50
FACS Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Product Note

This antibody recognizes the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2A).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human eIF-2-alpha.

Purification

Purified IgG

Conjugation

Unconjugated

RRID

AB_370417

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2 subunit alpha , EIF-2 , EIF-2A , EIF-2alpha , EIF2 , EIF2A

Background

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-eIF2 alpha antibody [EIF2a] used in IHC (Paraffin sections) (IHC-P). GTX25369

GTX25369 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX25369 eIF2 alpha antibody [EIF2a].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-eIF2 alpha antibody [EIF2a] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25369

GTX25369 ICC/IF Image

ICC/IF analysis of A549 cells using GTX25369 eIF2 alpha antibody [EIF2a]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in1% BSA and incubated for 3 hours at room temperature

Anti-eIF2 alpha antibody [EIF2a] used in IHC (Paraffin sections) (IHC-P). GTX25369

GTX25369 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX25369 eIF2 alpha antibody [EIF2a].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-eIF2 alpha antibody [EIF2a] used in Western Blot (WB). GTX25369

GTX25369 WB Image

WB analysis of whole cell extracts (20 μg lysate) of COS-7 (Lane 1), MCF7 (Lane 2), PC-12 (Lane 3), HeLa (lane 4), Jurkat (lane 5), NIH/3T3 (lane 6) and A-431 (lane 7) using GTX25369 eIF2 alpha antibody [EIF2a].
Dilution : 1:500-1:1000

REFERENCE

Application Reference

Wei PC et al. Nucleic Acids Res 2012; 40 (1) : 323-32 Non-targeting siRNA induces NPGPx expression to cooperate with exoribonuclease XRN2 for releasing the stress.
Application : WB
Submit a Reference

REVIEW

There are currently no reviews for eIF2 alpha antibody [EIF2a] (GTX25369). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399