GeneTex
  • Country / Location Selection

United States (US)

eIF2 alpha antibody [N1C1]

Anti-eIF2 alpha antibody [N1C1] used in Western Blot (WB). GTX112918
Anti-eIF2 alpha antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112918

Cat. No. GTX112918

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human eIF2 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950184

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2 subunit alpha , EIF-2 , EIF-2A , EIF-2alpha , EIF2 , EIF2A

Background

The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-eIF2 alpha antibody [N1C1] used in Western Blot (WB). GTX112918

GTX112918 WB Image

Sample (30 ug of whole cell lysate)

A: Hela

10% SDS PAGE

GTX112918 diluted at 1:2000

Anti-eIF2 alpha antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112918

GTX112918 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using eIF2 alpha(GTX112918) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for eIF2 alpha antibody [N1C1] (GTX112918). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for eIF2 alpha antibody [N1C1] (GTX112918). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169