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United States (US)

eIF2 alpha antibody [N1C3]

GTX101241_42774_20170323_WB_19011506_284.jpg
GTX101241_42809_20170712_IFA_19011506_677.jpg
GTX101241_42774_20170628_IFA_19011506_629.jpg

Cat No. GTX101241

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

A549 , H1299 , HCT-116 , *rat neuron , *HeLa , *N2a

Predict Reactivity

Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human eIF2 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Initiation Factor 2 Subunit Alpha,Eif-2,Eif-2A,Eif-2Alpha,Eif2,Eif2A,Eif2S1

Background

The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM]

Database

DATA IMAGES
GTX101241_42774_20170323_WB_19011506_284.jpg

GTX101241 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with eIF2 alpha antibody [N1C3] (GTX101241) diluted at 1:5000.

GTX101241_42809_20170712_IFA_19011506_677.jpg

GTX101241 ICC/IF Image

eIF2 alpha antibody [N1C3] detects eIF2 alpha protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: eIF2 alpha protein stained by eIF2 alpha antibody [N1C3] (GTX101241) diluted at 1:5000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX101241_42774_20170628_IFA_19011506_629.jpg

GTX101241 ICC/IF Image

eIF2 alpha antibody [N1C3] detects eIF2 alpha protein at cytoplasm by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: eIF2 alpha protein stained by eIF2 alpha antibody [N1C3] (GTX101241) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
REVIEW

eIF2 alpha antibody [N1C3] Cat No. GTX101241

Rating ( Average 3.8 based on 4 users reviews)
Application
Western Blot(WB) ( Average 3.8 based on 4 users reviews)
Date : Anonymous submitted on 30-Aug-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 20μg
Blocking : 5% Skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Date : Anonymous submitted on 30-Jun-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 10μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 30-Jun-2014
Score :
Application Tested : WB
Sample Species : Ms
Sample : N2a cells
Amount used : 10μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 16-Apr-2014
Score :
Application Tested : WB
Sample Species : Rat
Sample : rat neuron cells
Amount used : 10μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169