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eIF2 alpha antibody [N1C3]

Anti-eIF2 alpha antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101241
Anti-eIF2 alpha antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101241
Anti-eIF2 alpha antibody [N1C3] used in Western Blot (WB). GTX101241

Cat. No. GTX101241

Reference 7
5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all eIF2 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

A549 , H1299 , HCT-116

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human eIF2 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950186

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2 subunit alpha , EIF-2 , EIF-2A , EIF-2alpha , EIF2 , EIF2A

Background

The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-eIF2 alpha antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101241

GTX101241 ICC/IF Image

eIF2 alpha antibody [N1C3] detects eIF2 alpha protein at cytoplasm by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: eIF2 alpha protein stained by eIF2 alpha antibody [N1C3] (GTX101241) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-eIF2 alpha antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101241

GTX101241 ICC/IF Image

eIF2 alpha antibody [N1C3] detects eIF2 alpha protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: eIF2 alpha protein stained by eIF2 alpha antibody [N1C3] (GTX101241) diluted at 1:5000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-eIF2 alpha antibody [N1C3] used in Western Blot (WB). GTX101241

GTX101241 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with eIF2 alpha antibody [N1C3] (GTX101241) diluted at 1:5000.

REFERENCE

Application Reference

Wang IH et al. Cancers (Basel) 2020; 12 (5) Mevalonate Pathway Enzyme HMGCS1 Contributes to Gastric Cancer Progression.
Application : WB
Reactivity : Human
Wang L et al. J Hazard Mater 2019; : 121833 Activation of integrated stress response and disordered iron homeostasis upon combined exposure to cadmium and PCB77.
Application : WB
Reactivity : Human
Yen JH et al. Int J Mol Sci 2017; 18 (4) Fisetin Protects PC12 Cells from Tunicamycin-Mediated Cell Death via Reactive Oxygen Species Scavenging and Modulation of Nrf2-Driven Gene Expression, SIRT1 and MAPK Signaling in PC12 Cells.
Application : WB
Reactivity : Rat
Hsu HY et al. Sci Rep 2017; 7 : 44990 Fucoidan induces Toll-like receptor 4-regulated reactive oxygen species and promotes endoplasmic reticulum stress-mediated apoptosis in lung cancer.
Application : WB
Reactivity : Human
Wu PS et al. PLoS One 2015; 10 (6) : e0130599 Luteolin and Apigenin Attenuate 4-Hydroxy-2-Nonenal-Mediated Cell Death through Modulation of UPR, Nrf2-ARE and MAPK Pathways in PC12 Cells.
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REVIEW

eIF2 alpha antibody [N1C3] Cat. No. GTX101241

Rating
( Average 3.8 based on 5 users reviews)
Application
Western Blot(WB)
( Average 3.8 based on 5 users reviews)
Anti-eIF2 alpha antibody [N1C3] used in Western Blot (WB). GTX101241
Date : Anonymous submitted on 27-Jan-2022
Score :
Application Tested : WB
Sample Species : Ms
Sample : A10 cells
Amount used : 60μg
Blocking : 5% non-fat milk,BSA in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Anti-eIF2 alpha antibody [N1C3] used in Western Blot (WB). GTX101241
Date : Anonymous submitted on 30-Aug-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 20μg
Blocking : 5% Skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Anti-eIF2 alpha antibody [N1C3] used in Western Blot (WB). GTX101241
Date : Anonymous submitted on 30-Jun-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 10μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-eIF2 alpha antibody [N1C3] used in Western Blot (WB). GTX101241
Date : Anonymous submitted on 30-Jun-2014
Score :
Application Tested : WB
Sample Species : Ms
Sample : N2a cells
Amount used : 10μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-eIF2 alpha antibody [N1C3] used in Western Blot (WB). GTX101241
Date : Anonymous submitted on 16-Apr-2014
Score :
Application Tested : WB
Sample Species : Rat
Sample : rat neuron cells
Amount used : 10μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169