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eIF2 alpha (phospho Ser48) antibody

Anti-eIF2 alpha (phospho Ser48) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50342
Anti-eIF2 alpha (phospho Ser48) antibody used in IHC (Paraffin sections) (IHC-P). GTX50342
Anti-eIF2 alpha (phospho Ser48) antibody used in IHC (Paraffin sections) (IHC-P). GTX50342
Anti-eIF2 alpha (phospho Ser48) antibody used in Western Blot (WB). GTX50342

Cat. No. GTX50342

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all eIF2 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of Serine 49 (L-L-S(p)-E-L) derived from human eIF2 alpha.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2 subunit alpha , EIF-2 , EIF-2A , EIF-2alpha , EIF2 , EIF2A

Background

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-eIF2 alpha (phospho Ser48) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50342

GTX50342 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50342 eIF2 alpha (phospho Ser48) antibody.

Anti-eIF2 alpha (phospho Ser48) antibody used in IHC (Paraffin sections) (IHC-P). GTX50342

GTX50342 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50342 eIF2 alpha (phospho Ser48) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-eIF2 alpha (phospho Ser48) antibody used in IHC (Paraffin sections) (IHC-P). GTX50342

GTX50342 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX50342 eIF2 alpha (phospho Ser48) antibody.

Anti-eIF2 alpha (phospho Ser48) antibody used in Western Blot (WB). GTX50342

GTX50342 WB Image

WB analysis of extracts from HeLa cells untreated or treated with starvation using GTX50342 eIF2 alpha (phospho Ser48) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399