APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human Phospho-EIF2S1 (S51).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
eukaryotic translation initiation factor 2 subunit alpha , EIF2 , EIF2A , EIF2S1 , EIF2alpha , eIF2 alpha
Cellular Localization
Cytoplasmic granule
Background
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
Database
Research Area
DATA IMAGES
![Anti-eIF2 alpha (phospho Ser51) antibody [GT1203] used in Western Blot (WB). GTX00965](/templates/default/js/lightbox/images/loading.gif "Anti-eIF2 alpha (phospho Ser51) antibody [GT1203] used in Western Blot (WB). GTX00965")
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GTX00965 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with eIF2 alpha (phospho Ser51) antibody [GT1203] (GTX00965) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX00965 WB Image
WB analysis of HeLa cell extracts with/without Calyculin A (100 nM, 37ºC, 30 min) treatment after serum-starvation overnight or TNF-α (20 ng/ml, 37ºC, 30 min) treatment using GTX00965 eIF2 alpha (phospho Ser51) antibody [GT1203].
Dilution : 1:1000
Loading : 25μg
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GTX00965 WB Image
WB analysis of NIH-3T3 cell extracts with/without Calyculin A (100 nM, 37ºC, 30 min) treatment after serum-starvation overnight or TNF-α (20 ng/ml, 37ºC, 30 min) treatment using GTX00965 eIF2 alpha (phospho Ser51) antibody [GT1203].
Dilution : 1:1000
Loading : 25μg
|
REFERENCE
There are currently no references for eIF2 alpha (phospho Ser51) antibody [GT1203] (GTX00965). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for eIF2 alpha (phospho Ser51) antibody [GT1203] (GTX00965). Be the first to share your experience with this product.
![Anti-eIF2 alpha (phospho Ser51) antibody [GT1203] used in Western Blot (WB). GTX00965](/upload/website/prouct_img/small/GTX00965/GTX00965_4000000130_20200313_WB_treatment_Calyculin A_w_23053121_441.webp "Anti-eIF2 alpha (phospho Ser51) antibody [GT1203] used in Western Blot (WB). GTX00965")
![Anti-eIF2 alpha (phospho Ser51) antibody [GT1203] used in Western Blot (WB). GTX00965](/upload/website/prouct_img/small/GTX00965/GTX00965_20200327_WB_1_w_23053121_538.webp "Anti-eIF2 alpha (phospho Ser51) antibody [GT1203] used in Western Blot (WB). GTX00965")
![Anti-eIF2 alpha (phospho Ser51) antibody [GT1203] used in Western Blot (WB). GTX00965](/upload/website/prouct_img/small/GTX00965/GTX00965_20200327_WB_2_w_23053121_248.webp "Anti-eIF2 alpha (phospho Ser51) antibody [GT1203] used in Western Blot (WB). GTX00965")
![Anti-eIF2 alpha (phospho Ser51) antibody [GT1203] used in Western Blot (WB). GTX00965](/upload/website/prouct_img/normal/GTX00965/GTX00965_4000000130_20200313_WB_treatment_Calyculin A_w_23053121_441.webp "Anti-eIF2 alpha (phospho Ser51) antibody [GT1203] used in Western Blot (WB). GTX00965")
![Anti-eIF2 alpha (phospho Ser51) antibody [GT1203] used in Western Blot (WB). GTX00965](/upload/website/prouct_img/normal/GTX00965/GTX00965_20200327_WB_1_w_23053121_538.webp "Anti-eIF2 alpha (phospho Ser51) antibody [GT1203] used in Western Blot (WB). GTX00965")
![Anti-eIF2 alpha (phospho Ser51) antibody [GT1203] used in Western Blot (WB). GTX00965](/upload/website/prouct_img/normal/GTX00965/GTX00965_20200327_WB_2_w_23053121_248.webp "Anti-eIF2 alpha (phospho Ser51) antibody [GT1203] used in Western Blot (WB). GTX00965")