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eIF2 alpha (phospho Ser51) antibody

Orthogonal
Anti-eIF2 alpha (phospho Ser51) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24837
Anti-eIF2 alpha (phospho Ser51) antibody used in Western Blot (WB). GTX24837

Cat. No. GTX24837

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC (Free Floating)

Reactivity

 Human, Mouse, Rat, Yeast, Drosophila, Cat, Chicken, Caenorhabditis elegans, Primate
Package
50 μl ($399)
See all eIF2 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human eIF2a that contains serine 52. This region is conserved among many species including rat, pig, cow, fruit fly and yeast.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_370418

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2 subunit alpha , EIF-2 , EIF-2A , EIF-2alpha , EIF2 , EIF2A

Background

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-eIF2 alpha (phospho Ser51) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24837

GTX24837 ICC/IF Image

Rabbit anti EIF2alpha [pS51] phosphospecific polyclonal antibody.
Left: Image shows immunocytochemical analysis of EIF2alpha [pS51] in rat brain (mediodorsal neocortex) sections following traumatic axonal injury (TAI) (24 h). Control (prior to injury induction) shows low basal levels of EIF2alpha [pS51].
Right: Image shows double labeling of APP (red) and EIF2alpha [pS51] (green) in neurons subjected to traumatic axonal injury (TAI). Inset (above): higher magnification of neuronal soma with TAI. EIF2alpha [pS51] immunofluorescence is localized in cytoplasm of neuronal cell body.

Anti-eIF2 alpha (phospho Ser51) antibody used in Western Blot (WB). GTX24837

GTX24837 WB Image

Orthogonal

The data was published in the journal BMC Cancer in 2008. PMID: 18694499

REFERENCE

Application Reference

Yildirim AD et al. J Lipid Res 2022; 63 (10) : 100279 ER Stress-Induced Sphingosine-1-Phosphate Lyase Phosphorylation Potentiates the Mitochondrial Unfolded Protein Response.
Application : WB
Reactivity : Human, Mouse
Jorgensen E et al. BMC Cancer 2008; 8 : 229 Cigarette smoke induces endoplasmic reticulum stress and the unfolded protein response in normal and malignant human lung cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399