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eIF2 alpha (phospho Ser51) antibody

Orthogonal
Anti-eIF2 alpha (phospho Ser51) antibody used in IHC (Paraffin sections) (IHC-P). GTX50300
Anti-eIF2 alpha (phospho Ser51) antibody used in Western Blot (WB). GTX50300
Anti-eIF2 alpha (phospho Ser51) antibody used in Western Blot (WB). GTX50300
Anti-eIF2 alpha (phospho Ser51) antibody used in Western Blot (WB). GTX50300

Cat. No. GTX50300

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Monkey
Package
100 μl ($399)
See all eIF2 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human eIF2α around the phosphorylation site of serine 51 (E-L-Sp-R-R).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11164559

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EIF2,EIF2A,EIF2S1,EIF2alpha,eukaryotic translation initiation factor 2 subunit alpha,eIF2 alpha

Cellular Localization

Cytoplasmic granule

Background

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-eIF2 alpha (phospho Ser51) antibody used in IHC (Paraffin sections) (IHC-P). GTX50300

GTX50300 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50300 eIF2 alpha (phospho Ser51) antibody.

Anti-eIF2 alpha (phospho Ser51) antibody used in Western Blot (WB). GTX50300

GTX50300 WB Image

Orthogonal

WB analysis of K562 cells untreated or treated with IFN-α (100ng/ml, 20min) lysates using GTX50300 eIF2 alpha (phospho Ser51) antibody (Lane 3 and 4) and eIF2α antibody (Lane 1 and 2).

Anti-eIF2 alpha (phospho Ser51) antibody used in Western Blot (WB). GTX50300

GTX50300 WB Image

Orthogonal

The data was published in the journal Viruses in 2019.PMID: 31216693

Anti-eIF2 alpha (phospho Ser51) antibody used in Western Blot (WB). GTX50300

GTX50300 WB Image

The data was published in the 2022 in Orphanet J Rare Dis. PMID: 35101074

REFERENCE

Application Reference

Arlt A et al. Orphanet J Rare Dis 2022; 17 (1) : 29 Novel insights into PORCN mutations, associated phenotypes and pathophysiological aspects.
Application : WB
Reactivity : Human
Lutz MM 4th et al. Viruses 2019; 11 (6) Mammalian orthoreovirus Infection is Enhanced in Cells Pre-Treated with Sodium Arsenite.
Lee KH et al. Sci Rep 2014; 4 : 6394 A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.
Application : WB
Reactivity : Human
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Package List Price ($)
$ 399