GeneTex
  • Country / Location Selection

United States (US)

eIF2 alpha (phospho Ser51) antibody

Anti-eIF2 alpha (phospho Ser51) antibody used in Immunoprecipitation (IP). GTX66600
Anti-eIF2 alpha (phospho Ser51) antibody used in Western Blot (WB). GTX66600

Cat No. GTX66600

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
IP 1:50-1:100
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic phosphorylated peptide around S51 of human eIF2α (NP_004085.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation initiation factor 2 subunit alpha , EIF-2 , EIF-2A , EIF-2alpha , EIF2 , EIF2A

Background

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-eIF2 alpha (phospho Ser51) antibody used in Immunoprecipitation (IP). GTX66600

GTX66600 IP Image

IP analysis of HeLa cells treated by Calyculin A (100 nM) at 37ºC for 30 minutes after serum-starvation overnight using GTX66600 eIF2 alpha (phospho Ser51) antibody.
IP reaction : 200μg extracts / 3μg antibody antibody
Dilution : 1:1000

Anti-eIF2 alpha (phospho Ser51) antibody used in Western Blot (WB). GTX66600

GTX66600 WB Image

WB analysis of HeLa treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight and Jurkat cell treated by Calyculin A (100nM) for 30 minutes using GTX66600 eIF2 alpha (phospho Ser51) antibody.
Loading : 25ug per lane
Dilution : 1:2000

REFERENCE

There are currently no references for eIF2 alpha (phospho Ser51) antibody (GTX66600). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for eIF2 alpha (phospho Ser51) antibody (GTX66600). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf