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eIF2 alpha (phospho Ser52) antibody

Anti-eIF2 alpha (phospho Ser52) antibody used in Western Blot (WB). GTX130006
Anti-eIF2 alpha (phospho Ser52) antibody used in Western Blot (WB). GTX130006
Anti-eIF2 alpha (phospho Ser52) antibody used in Western Blot (WB). GTX130006
Anti-eIF2 alpha (phospho Ser52) antibody used in Western Blot (WB). GTX130006

Cat No. GTX130006

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , 293T (25 μg/ml anisomycin treatment for 16hr) , HeLa , HeLa (4 μg/ml Tunicamycin treatment for 16hr) , HepG2 , HepG2 (3 μM Thapsigargin treatment for 12 hr) , PC-12 (300 μM Thapsigargin treatment for 8hr)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human eIF2 alpha (phospho Ser52). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation initiation factor 2 subunit alpha , EIF-2 , EIF-2A , EIF-2alpha , EIF2 , EIF2A

Background

The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM]

Database

DATA IMAGES

Anti-eIF2 alpha (phospho Ser52) antibody used in Western Blot (WB). GTX130006

GTX130006 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EIF2 alpha (phospho Ser52) antibody (GTX130006) diluted at 1:500.

Anti-eIF2 alpha (phospho Ser52) antibody used in Western Blot (WB). GTX130006

GTX130006 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EIF2 alpha (phospho Ser52) antibody (GTX130006) diluted at 1:500.

Anti-eIF2 alpha (phospho Ser52) antibody used in Western Blot (WB). GTX130006

GTX130006 WB Image

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EIF2 alpha (phospho Ser52) antibody (GTX130006) diluted at 1:500.

Anti-eIF2 alpha (phospho Ser52) antibody used in Western Blot (WB). GTX130006

GTX130006 WB Image

EIF2 alpha (phospho Ser52) antibody detects EIF2 alpha (phospho Ser52) protein by western blot analysis. Un-treated (-) and treated (+, 300 μM Thapsigargin treatment for 8hr) PC-12 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EIF2 alpha (phospho Ser52) antibody (GTX130006) at a dilution of 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169