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eIF2 alpha (phospho Ser51) antibody

Orthogonal
Anti-eIF2 alpha (phospho Ser51) antibody used in Western Blot (WB). GTX130006
Anti-eIF2 alpha (phospho Ser51) antibody used in Western Blot (WB). GTX130006
Anti-eIF2 alpha (phospho Ser51) antibody used in Western Blot (WB). GTX130006

Cat. No. GTX130006

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all eIF2 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

PC-12 (300 μM Thapsigargin treatment for 8hr) , 293T (25 μg/ml anisomycin treatment for 16hr)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser52 of human eIF2 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886153

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2 subunit alpha , EIF-2 , EIF-2A , EIF-2alpha , EIF2 , EIF2A

Background

The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-eIF2 alpha (phospho Ser51) antibody used in Western Blot (WB). GTX130006

GTX130006 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with eIF2 alpha (phospho Ser51) antibody (GTX130006) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-eIF2 alpha (phospho Ser51) antibody used in Western Blot (WB). GTX130006

GTX130006 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EIF2 alpha (phospho Ser51) antibody (GTX130006) diluted at 1:500.

Anti-eIF2 alpha (phospho Ser51) antibody used in Western Blot (WB). GTX130006

GTX130006 WB Image

Orthogonal

EIF2 alpha (phospho Ser52) antibody detects EIF2 alpha (phospho Ser51) protein by western blot analysis. Un-treated (-) and treated (+, 300 μM Thapsigargin treatment for 8hr) PC-12 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EIF2 alpha (phospho Ser52) antibody (GTX130006) at a dilution of 1:1000.

REFERENCE

Application Reference

Wang IH et al. Cancers (Basel) 2020; 12 (5) Mevalonate Pathway Enzyme HMGCS1 Contributes to Gastric Cancer Progression.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169
Currently in production