IHC-P analysis of human breast tissue using GTX54741 eIF2B3 antibody [1H3].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

IP analysis of U2OS cell lysates using GTX54741 eIF2B3 antibody [1H3].
IP reaction : 3μg antibody / 500μg lysate
Dilution : 1:1000

ICC/IF analysis of COS7 cells using GTX54741 eIF2B3 antibody [1H3]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

WB analysis of 80μg whole cell lysate using GTX54741 eIF2B3 antibody [1H3].
Dilution : 1:1000

ICC/IF analysis of HeLa cells using GTX54741 eIF2B3 antibody [1H3]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

IHC-P analysis of human uterus tissue using GTX54741 eIF2B3 antibody [1H3].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100