APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
A431
Predict Reactivity
Mouse, Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human eIF3H / EIF3S3. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888354
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
eukaryotic translation initiation factor 3 subunit H , EIF3S3 , eIF3-gamma , eIF3-p40
Cellular Localization
Cytoplasm
Background
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).
Database
Research Area
DATA IMAGES
![Anti-eIF3H / EIF3S3 antibody [GT2712] used in Western Blot (WB). GTX633630](/templates/default/js/lightbox/images/loading.gif "Anti-eIF3H / EIF3S3 antibody [GT2712] used in Western Blot (WB). GTX633630")
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GTX633630 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with eIF3H / EIF3S3 antibody [GT2712] (GTX633630) diluted at 1:1000.
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REFERENCE
There are currently no references for eIF3H / EIF3S3 antibody [GT2712] (GTX633630). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for eIF3H / EIF3S3 antibody [GT2712] (GTX633630). Be the first to share your experience with this product.
![Anti-eIF3H / EIF3S3 antibody [GT2712] used in Western Blot (WB). GTX633630](/upload/website/prouct_img/small/GTX633630/GTX633630_42583_20160922_WB_w_23061202_982.webp "Anti-eIF3H / EIF3S3 antibody [GT2712] used in Western Blot (WB). GTX633630")
![Anti-eIF3H / EIF3S3 antibody [GT2712] used in Western Blot (WB). GTX633630](/upload/website/prouct_img/normal/GTX633630/GTX633630_42583_20160922_WB_w_23061202_982.webp "Anti-eIF3H / EIF3S3 antibody [GT2712] used in Western Blot (WB). GTX633630")