*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Rat, Bovine, Chicken, Xenopus laevis(>80% identity)
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human eIF3H / EIF3S3. The exact sequence is proprietary.
Affinity purified by Protein A.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
eukaryotic translation initiation factor 3 subunit H , EIF3S3 , eIF3-gamma , eIF3-p40
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).
GTX633631 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with eIF3H / EIF3S3 antibody [GT35512] (GTX633631) diluted at 1:1000.
GTX633631 ICC/IF Image
eIF3H / EIF3S3 antibody [GT35512] detects eIF3H / EIF3S3 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: eIF3H / EIF3S3 protein stained by eIF3H / EIF3S3 antibody [GT35512] (GTX633631) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
GTX633631 IHC-P Image
eIF3H / EIF3S3 antibody [GT35512] detects eIF3H / EIF3S3 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
eIF3H / EIF3S3 antibody [GT35512] (GTX633631) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min