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eIF3H / EIF3S3 antibody [GT35512]

Anti-eIF3H / EIF3S3 antibody [GT35512] used in Western Blot (WB). GTX633631
Anti-eIF3H / EIF3S3 antibody [GT35512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633631
Anti-eIF3H / EIF3S3 antibody [GT35512] used in IHC (Paraffin sections) (IHC-P). GTX633631

Cat No. GTX633631

Host

Mouse

Clonality

Monoclonal

Clone Name

GT35512

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human eIF3H / EIF3S3. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888355

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation initiation factor 3 subunit H , EIF3S3 , eIF3-gamma , eIF3-p40

Cellular Localization

Cytoplasm

Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).

Database

DATA IMAGES

Anti-eIF3H / EIF3S3 antibody [GT35512] used in Western Blot (WB). GTX633631

GTX633631 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with eIF3H / EIF3S3 antibody [GT35512] (GTX633631) diluted at 1:1000.

Anti-eIF3H / EIF3S3 antibody [GT35512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633631

GTX633631 ICC/IF Image

eIF3H / EIF3S3 antibody [GT35512] detects eIF3H / EIF3S3 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: eIF3H / EIF3S3 protein stained by eIF3H / EIF3S3 antibody [GT35512] (GTX633631) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-eIF3H / EIF3S3 antibody [GT35512] used in IHC (Paraffin sections) (IHC-P). GTX633631

GTX633631 IHC-P Image

eIF3H / EIF3S3 antibody [GT35512] detects eIF3H / EIF3S3 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
eIF3H / EIF3S3 antibody [GT35512] (GTX633631) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for eIF3H / EIF3S3 antibody [GT35512] (GTX633631). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for eIF3H / EIF3S3 antibody [GT35512] (GTX633631). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169