APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji
Predict Reactivity
Mouse, Rat, Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.87mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human EIF3 gamma. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
EIF3S3 antibody, MGC102958 antibody, eIF3-gamma antibody, eIF3-p40 antibody, EIF3H antibody, eukaryotic translation initiation factor 3 subunit 3 antibody, eIF-3-gamma antibody, eukaryotic translation initiation factor 3 subunit H antibody, "eukaryotic translation initiation factor 3, subunit 3 gamma, 40kDa antibody", "eukaryotic translation initiation factor 3, subunit 3 (gamma, 40kD) antibody", "eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD) antibody", eIF3 p40 subunit antibody, "eukaryotic translation initiation factor 3, subunit H antibody"
Cellular Localization
Cytoplasm
Background
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Database
DATA IMAGES
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GTX106886 WB Image
Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:H1299
C:Hep G2 (GTX27900)
10% SDS PAGE
GTX106886 diluted at 1:1000
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