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eIF4B antibody [1F5]

Anti-eIF4B antibody [1F5] used in Western Blot (WB). GTX60596
Anti-eIF4B antibody [1F5] used in Western Blot (WB). GTX60596
Anti-eIF4B antibody [1F5] used in Flow cytometry (FACS). GTX60596
Anti-eIF4B antibody [1F5] used in IHC (Paraffin sections) (IHC-P). GTX60596
Anti-eIF4B antibody [1F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60596
Anti-eIF4B antibody [1F5] used in ELISA (ELISA). GTX60596

Cat. No. GTX60596

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1F5

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($409)
See all eIF4B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human EIF4B expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 4B , EIF-4B , PRO1843

Database

Research Area

DATA IMAGES

Anti-eIF4B antibody [1F5] used in Western Blot (WB). GTX60596

GTX60596 WB Image

WB analysis of A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate using GTX60596 eIF4B antibody [1F5].

Anti-eIF4B antibody [1F5] used in Western Blot (WB). GTX60596

GTX60596 WB Image

WB analysis of human EIF4B (AA: 381-585) recombinant protein using GTX60596 eIF4B antibody [1F5].

Anti-eIF4B antibody [1F5] used in Flow cytometry (FACS). GTX60596

GTX60596 FACS Image

FACS analysis of HeLa cells using GTX60596 eIF4B antibody [1F5].
Green : eIF4B
Red : negative control

Anti-eIF4B antibody [1F5] used in IHC (Paraffin sections) (IHC-P). GTX60596

GTX60596 IHC-P Image

IHC-P analysis of colon cancer tissue using GTX60596 eIF4B antibody [1F5].

Anti-eIF4B antibody [1F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60596

GTX60596 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using GTX60596 eIF4B antibody [1F5].
Green : eIF4B
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-eIF4B antibody [1F5] used in ELISA (ELISA). GTX60596

GTX60596 ELISA Image

ELISA analysis of antigen using GTX60596 eIF4B antibody [1F5].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409