APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
NT2D1 , IMR32 , U87-MG , MCF-7 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , human EIF4B-transfected 293T , 293T , A431 , HeLa , HepG2
Predict Reactivity
Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.16 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human eIF4B. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10721705
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
eukaryotic translation initiation factor 4B , EIF-4B , PRO1843
Background
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Database
Research Area
DATA IMAGES
![Anti-eIF4B antibody [N1N2], N-term used in Western Blot (WB). GTX112612](/templates/default/js/lightbox/images/loading.gif "Anti-eIF4B antibody [N1N2], N-term used in Western Blot (WB). GTX112612")
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GTX112612 WB Image
EIF4B antibody detects EIF4B protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of human EIF4B-transfected 293T cells
7.5 % SDS-PAGE
EIF4B antibody (GTX112612) dilution: 1:5000
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GTX112612 WB Image
EIF4B antibody detects EIF4B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EIF4B antibody (GTX112612) diluted by 1:1000.
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GTX112612 WB Image
EIF4B antibody detects EIF4B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5 % SDS-PAGE, and blotted with EIF4B antibody (GTX112612) diluted by 1:1000
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GTX112612 IHC-P Image
EIF4B antibody [N1N2], N-term detects EIF4B protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
EIF4B antibody [N1N2], N-term (GTX112612) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX112612 WB Image
Sample (30 ug of whole cell lysate)
A: NT2D1
B: IMR32
C: U87-MG
D: MCF-7
7.5% SDS PAGE
GTX112612 diluted at 1:1000
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GTX112612 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using EIF4B(GTX112612) antibody at 1:500 dilution.
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REFERENCE
There are currently no references for eIF4B antibody [N1N2], N-term (GTX112612). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for eIF4B antibody [N1N2], N-term (GTX112612). Be the first to share your experience with this product.
![Anti-eIF4B antibody [N1N2], N-term used in Western Blot (WB). GTX112612](/upload/website/prouct_img/small/GTX112612/GTX112612_40485_WB_B_w_23060500_240.webp "Anti-eIF4B antibody [N1N2], N-term used in Western Blot (WB). GTX112612")
![Anti-eIF4B antibody [N1N2], N-term used in Western Blot (WB). GTX112612](/upload/website/prouct_img/small/GTX112612/GTX112612_40485_WB_3_w_23060500_669.webp "Anti-eIF4B antibody [N1N2], N-term used in Western Blot (WB). GTX112612")
![Anti-eIF4B antibody [N1N2], N-term used in Western Blot (WB). GTX112612](/upload/website/prouct_img/small/GTX112612/GTX112612_40485_WB_2_w_23060500_282.webp "Anti-eIF4B antibody [N1N2], N-term used in Western Blot (WB). GTX112612")
![Anti-eIF4B antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112612](/upload/website/prouct_img/small/GTX112612/GTX112612_41087_IHC_M_w_23060500_895.webp "Anti-eIF4B antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112612")
![Anti-eIF4B antibody [N1N2], N-term used in Western Blot (WB). GTX112612](/upload/website/prouct_img/small/GTX112612/GTX112612_40485_WB_w_23060500_764.webp "Anti-eIF4B antibody [N1N2], N-term used in Western Blot (WB). GTX112612")
![Anti-eIF4B antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112612](/upload/website/prouct_img/small/GTX112612/GTX112612_40485_IFA_w_23060500_399.webp "Anti-eIF4B antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112612")
![Anti-eIF4B antibody [N1N2], N-term used in Western Blot (WB). GTX112612](/upload/website/prouct_img/normal/GTX112612/GTX112612_40485_WB_B_w_23060500_240.webp "Anti-eIF4B antibody [N1N2], N-term used in Western Blot (WB). GTX112612")
![Anti-eIF4B antibody [N1N2], N-term used in Western Blot (WB). GTX112612](/upload/website/prouct_img/normal/GTX112612/GTX112612_40485_WB_3_w_23060500_669.webp "Anti-eIF4B antibody [N1N2], N-term used in Western Blot (WB). GTX112612")
![Anti-eIF4B antibody [N1N2], N-term used in Western Blot (WB). GTX112612](/upload/website/prouct_img/normal/GTX112612/GTX112612_40485_WB_2_w_23060500_282.webp "Anti-eIF4B antibody [N1N2], N-term used in Western Blot (WB). GTX112612")
![Anti-eIF4B antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112612](/upload/website/prouct_img/normal/GTX112612/GTX112612_41087_IHC_M_w_23060500_895.webp "Anti-eIF4B antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112612")
![Anti-eIF4B antibody [N1N2], N-term used in Western Blot (WB). GTX112612](/upload/website/prouct_img/normal/GTX112612/GTX112612_40485_WB_w_23060500_764.webp "Anti-eIF4B antibody [N1N2], N-term used in Western Blot (WB). GTX112612")
![Anti-eIF4B antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112612](/upload/website/prouct_img/normal/GTX112612/GTX112612_40485_IFA_w_23060500_399.webp "Anti-eIF4B antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112612")