APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human EIF4E expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
eukaryotic translation initiation factor 4E , AUTS19 , CBP , EIF4E1 , EIF4EL1 , EIF4F , eIF-4E
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Database
Research Area
DATA IMAGES
![Anti-eIF4E antibody [5D11] used in Flow cytometry (FACS). GTX60420](/templates/default/js/lightbox/images/loading.gif "Anti-eIF4E antibody [5D11] used in Flow cytometry (FACS). GTX60420")
|
GTX60420 FACS Image
FACS analysis of HeLa cells using GTX60420 eIF4E antibody [5D11].
Green : eIF4E
Purple : negative control
|
|
|
GTX60420 ELISA Image
ELISA analysis of antigen using GTX60420 eIF4E antibody [5D11].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng
|
|
|
GTX60420 IHC-P Image
IHC-P analysis of liver cancer (left) and submaxillary tumor (right) using GTX60420 eIF4E antibody [5D11].
|
|
|
GTX60420 ICC/IF Image
ICC/IF analysis of GC-7901 cells using GTX60420 eIF4E antibody [5D11].
Green : eIF4E
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
|
|
|
GTX60420 WB Image
WB analysis of HeLa (1), HEK293 (2) and K562 (3) cell lysate using GTX60420 eIF4E antibody [5D11].
|
|
|
GTX60420 IHC-P Image
IHC-P analysis of stomach cancer (left) and prostate cancer (right) using GTX60420 eIF4E antibody [5D11].
|
REFERENCE
There are currently no references for eIF4E antibody [5D11] (GTX60420). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for eIF4E antibody [5D11] (GTX60420). Be the first to share your experience with this product.
![Anti-eIF4E antibody [5D11] used in Flow cytometry (FACS). GTX60420](/upload/website/prouct_img/small/GTX60420/GTX60420_20170912_FACS_18121411_923.jpg "Anti-eIF4E antibody [5D11] used in Flow cytometry (FACS). GTX60420")
![Anti-eIF4E antibody [5D11] used in ELISA (ELISA). GTX60420](/upload/website/prouct_img/small/GTX60420/GTX60420_20170912_ELISA_18121411_815.jpg "Anti-eIF4E antibody [5D11] used in ELISA (ELISA). GTX60420")
![Anti-eIF4E antibody [5D11] used in IHC (Paraffin sections) (IHC-P). GTX60420](/upload/website/prouct_img/small/GTX60420/GTX60420_20170912_IHC-P_18121411_323.jpg "Anti-eIF4E antibody [5D11] used in IHC (Paraffin sections) (IHC-P). GTX60420")
![Anti-eIF4E antibody [5D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60420](/upload/website/prouct_img/small/GTX60420/GTX60420_20170912_ICCIF_18121411_770.jpg "Anti-eIF4E antibody [5D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60420")
![Anti-eIF4E antibody [5D11] used in Western Blot (WB). GTX60420](/upload/website/prouct_img/small/GTX60420/GTX60420_20170912_WB_18121411_180.jpg "Anti-eIF4E antibody [5D11] used in Western Blot (WB). GTX60420")
![Anti-eIF4E antibody [5D11] used in IHC (Paraffin sections) (IHC-P). GTX60420](/upload/website/prouct_img/small/GTX60420/GTX60420_20170912_IHC-P_1_18121411_995.jpg "Anti-eIF4E antibody [5D11] used in IHC (Paraffin sections) (IHC-P). GTX60420")
![Anti-eIF4E antibody [5D11] used in Flow cytometry (FACS). GTX60420](/upload/website/prouct_img/normal/GTX60420/GTX60420_20170912_FACS_18121411_923.jpg "Anti-eIF4E antibody [5D11] used in Flow cytometry (FACS). GTX60420")
![Anti-eIF4E antibody [5D11] used in ELISA (ELISA). GTX60420](/upload/website/prouct_img/normal/GTX60420/GTX60420_20170912_ELISA_18121411_815.jpg "Anti-eIF4E antibody [5D11] used in ELISA (ELISA). GTX60420")
![Anti-eIF4E antibody [5D11] used in IHC (Paraffin sections) (IHC-P). GTX60420](/upload/website/prouct_img/normal/GTX60420/GTX60420_20170912_IHC-P_18121411_323.jpg "Anti-eIF4E antibody [5D11] used in IHC (Paraffin sections) (IHC-P). GTX60420")
![Anti-eIF4E antibody [5D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60420](/upload/website/prouct_img/normal/GTX60420/GTX60420_20170912_ICCIF_18121411_770.jpg "Anti-eIF4E antibody [5D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60420")
![Anti-eIF4E antibody [5D11] used in Western Blot (WB). GTX60420](/upload/website/prouct_img/normal/GTX60420/GTX60420_20170912_WB_18121411_180.jpg "Anti-eIF4E antibody [5D11] used in Western Blot (WB). GTX60420")
![Anti-eIF4E antibody [5D11] used in IHC (Paraffin sections) (IHC-P). GTX60420](/upload/website/prouct_img/normal/GTX60420/GTX60420_20170912_IHC-P_1_18121411_995.jpg "Anti-eIF4E antibody [5D11] used in IHC (Paraffin sections) (IHC-P). GTX60420")