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eIF4E antibody [5D11]

Anti-eIF4E antibody [5D11] used in Flow cytometry (FACS). GTX60420
Anti-eIF4E antibody [5D11] used in ELISA (ELISA). GTX60420
Anti-eIF4E antibody [5D11] used in IHC (Paraffin sections) (IHC-P). GTX60420
Anti-eIF4E antibody [5D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60420
Anti-eIF4E antibody [5D11] used in Western Blot (WB). GTX60420
Anti-eIF4E antibody [5D11] used in IHC (Paraffin sections) (IHC-P). GTX60420

Cat. No. GTX60420

Host

Mouse

Clonality

Monoclonal

Clone Name

5D11

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

Human
Package
100 μl ($409)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human EIF4E expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation initiation factor 4E , AUTS19 , CBP , EIF4E1 , EIF4EL1 , EIF4F , eIF-4E

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-eIF4E antibody [5D11] used in Flow cytometry (FACS). GTX60420

GTX60420 FACS Image

FACS analysis of HeLa cells using GTX60420 eIF4E antibody [5D11].
Green : eIF4E
Purple : negative control

Anti-eIF4E antibody [5D11] used in ELISA (ELISA). GTX60420

GTX60420 ELISA Image

ELISA analysis of antigen using GTX60420 eIF4E antibody [5D11].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-eIF4E antibody [5D11] used in IHC (Paraffin sections) (IHC-P). GTX60420

GTX60420 IHC-P Image

IHC-P analysis of liver cancer (left) and submaxillary tumor (right) using GTX60420 eIF4E antibody [5D11].

Anti-eIF4E antibody [5D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60420

GTX60420 ICC/IF Image

ICC/IF analysis of GC-7901 cells using GTX60420 eIF4E antibody [5D11].
Green : eIF4E
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-eIF4E antibody [5D11] used in Western Blot (WB). GTX60420

GTX60420 WB Image

WB analysis of HeLa (1), HEK293 (2) and K562 (3) cell lysate using GTX60420 eIF4E antibody [5D11].

Anti-eIF4E antibody [5D11] used in IHC (Paraffin sections) (IHC-P). GTX60420

GTX60420 IHC-P Image

IHC-P analysis of stomach cancer (left) and prostate cancer (right) using GTX60420 eIF4E antibody [5D11].

REFERENCE

There are currently no references for eIF4E antibody [5D11] (GTX60420). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for eIF4E antibody [5D11] (GTX60420). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 409