eIF4E antibody [5D11]

Cat. No. GTX60420

Host	Mouse
Clonality	Monoclonal
Clone Name	5D11
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human
Package	100 μl ($409)

See all eIF4E products

Datasheet
Document

View product citations for antibody GTX60420 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human EIF4E expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 4E , AUTS19 , CBP , EIF4E1 , EIF4EL1 , EIF4F , eIF-4E

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Gene ID: 1977 EIF4E
UniProt: P06730 EIF4E

Research Area

DATA IMAGES

GTX60420 WB Image

WB analysis of HeLa (1), HEK293 (2) and K562 (3) cell lysate using GTX60420 eIF4E antibody [5D11].

GTX60420 IHC-P Image

IHC-P analysis of stomach cancer (left) and prostate cancer (right) using GTX60420 eIF4E antibody [5D11].

GTX60420 ICC/IF Image

ICC/IF analysis of GC-7901 cells using GTX60420 eIF4E antibody [5D11].
Green : eIF4E
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

GTX60420 IHC-P Image

IHC-P analysis of liver cancer (left) and submaxillary tumor (right) using GTX60420 eIF4E antibody [5D11].

GTX60420 FACS Image

FACS analysis of HeLa cells using GTX60420 eIF4E antibody [5D11].
Green : eIF4E
Purple : negative control

GTX60420 ELISA Image

ELISA analysis of antigen using GTX60420 eIF4E antibody [5D11].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 409

Qty

eIF4E antibody [5D11]

Cat. No. GTX60420

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX60420 WB Image

GTX60420 IHC-P Image

GTX60420 ICC/IF Image

GTX60420 IHC-P Image

GTX60420 FACS Image

GTX60420 ELISA Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.