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eIF4E antibody [HL1553]

Recombinant antibody Orthogonal
Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028
Anti-eIF4E antibody [HL1553] used in IHC (Paraffin sections) (IHC-P). GTX637028
Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028
Anti-eIF4E antibody [HL1553] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637028
Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028
Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028
Anti-eIF4E antibody [HL1553] used in IHC (Paraffin sections) (IHC-P). GTX637028

Cat. No. GTX637028

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1553

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all eIF4E products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human eIF4E. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 4E , AUTS19 , CBP , EIF4E1 , EIF4EL1 , EIF4F , eIF-4E

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028

GTX637028 WB Image

Orthogonal

293T whole cell and cytoplasm extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1553] (GTX637028) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-eIF4E antibody [HL1553] used in IHC (Paraffin sections) (IHC-P). GTX637028

GTX637028 IHC-P Image

eIF4E antibody [HL1553] detects eIF4E protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
eIF4E stained by eIF4E antibody [HL1553] (GTX637028) diluted at 1:100.
Antigen Retrieval: Tris-EDTA buffer, pH 8.0, 15 min

Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028

GTX637028 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1553] (GTX637028) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-eIF4E antibody [HL1553] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637028

GTX637028 ICC/IF Image

eIF4E antibody [HL1553] detects eIF4E protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: eIF4E stained by eIF4E antibody [HL1553] (GTX637028) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028

GTX637028 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1553] (GTX637028) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028

GTX637028 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1553] (GTX637028) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-eIF4E antibody [HL1553] used in IHC (Paraffin sections) (IHC-P). GTX637028

GTX637028 IHC-P Image

eIF4E antibody [HL1553] detects eIF4E protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
eIF4E stained by eIF4E antibody [HL1553] (GTX637028) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations