APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human eIF4E. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
eukaryotic translation initiation factor 4E , AUTS19 , CBP , EIF4E1 , EIF4EL1 , EIF4F , eIF-4E
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Database
Research Area
DATA IMAGES
![Anti-eIF4E antibody [HL1554] used in Western Blot (WB). GTX637029](/templates/default/js/lightbox/images/loading.gif "Anti-eIF4E antibody [HL1554] used in Western Blot (WB). GTX637029")
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GTX637029 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1554] (GTX637029) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637029 IHC-P Image
eIF4E antibody [HL1554] detects eIF4E protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.eIF4E stained by eIF4E antibody [HL1554] (GTX637029) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637029 WB Image
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1554] (GTX637029) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637029 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1554] (GTX637029) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for eIF4E antibody [HL1554] (GTX637029). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for eIF4E antibody [HL1554] (GTX637029). Be the first to share your experience with this product.
![Anti-eIF4E antibody [HL1554] used in Western Blot (WB). GTX637029](/upload/website/prouct_img/small/GTX637029/GTX637029_44753_20220729_WB_22080119_604.webp "Anti-eIF4E antibody [HL1554] used in Western Blot (WB). GTX637029")
![Anti-eIF4E antibody [HL1554] used in IHC (Paraffin sections) (IHC-P). GTX637029](/upload/website/prouct_img/small/GTX637029/GTX637029_44753_20220819_IHC-P_M_22090701_844.webp "Anti-eIF4E antibody [HL1554] used in IHC (Paraffin sections) (IHC-P). GTX637029")
![Anti-eIF4E antibody [HL1554] used in Western Blot (WB). GTX637029](/upload/website/prouct_img/small/GTX637029/GTX637029_T-44697_20220610_WB_M_testis_22061601_601.jpg "Anti-eIF4E antibody [HL1554] used in Western Blot (WB). GTX637029")
![Anti-eIF4E antibody [HL1554] used in Western Blot (WB). GTX637029](/upload/website/prouct_img/small/GTX637029/GTX637029_T-44697_20220617_WB_M_R_22062121_206.webp "Anti-eIF4E antibody [HL1554] used in Western Blot (WB). GTX637029")
![Anti-eIF4E antibody [HL1554] used in Western Blot (WB). GTX637029](/upload/website/prouct_img/normal/GTX637029/GTX637029_44753_20220729_WB_22080119_604.webp "Anti-eIF4E antibody [HL1554] used in Western Blot (WB). GTX637029")
![Anti-eIF4E antibody [HL1554] used in IHC (Paraffin sections) (IHC-P). GTX637029](/upload/website/prouct_img/normal/GTX637029/GTX637029_44753_20220819_IHC-P_M_22090701_844.webp "Anti-eIF4E antibody [HL1554] used in IHC (Paraffin sections) (IHC-P). GTX637029")
![Anti-eIF4E antibody [HL1554] used in Western Blot (WB). GTX637029](/upload/website/prouct_img/normal/GTX637029/GTX637029_T-44697_20220610_WB_M_testis_22061601_601.jpg "Anti-eIF4E antibody [HL1554] used in Western Blot (WB). GTX637029")
![Anti-eIF4E antibody [HL1554] used in Western Blot (WB). GTX637029](/upload/website/prouct_img/normal/GTX637029/GTX637029_T-44697_20220617_WB_M_R_22062121_206.webp "Anti-eIF4E antibody [HL1554] used in Western Blot (WB). GTX637029")