Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1554] (GTX637029) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

eIF4E antibody [HL1554] detects eIF4E protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
eIF4E stained by eIF4E antibody [HL1554] (GTX637029) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1554] (GTX637029) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1554] (GTX637029) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.