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eIF4E antibody [HL1555]

Recombinant antibody
Anti-eIF4E antibody [HL1555] used in Western Blot (WB). GTX637030
Anti-eIF4E antibody [HL1555] used in Western Blot (WB). GTX637030
Anti-eIF4E antibody [HL1555] used in IHC (Paraffin sections) (IHC-P). GTX637030

Cat. No. GTX637030

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1555

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human eIF4E. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation initiation factor 4E , AUTS19 , CBP , EIF4E1 , EIF4EL1 , EIF4F , eIF-4E

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-eIF4E antibody [HL1555] used in Western Blot (WB). GTX637030

GTX637030 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1555] (GTX637030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-eIF4E antibody [HL1555] used in Western Blot (WB). GTX637030

GTX637030 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1555] (GTX637030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-eIF4E antibody [HL1555] used in IHC (Paraffin sections) (IHC-P). GTX637030

GTX637030 IHC-P Image

eIF4E antibody [HL1555] detects eIF4E protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
eIF4E stained by eIF4E antibody [HL1555] (GTX637030) diluted at 1:100.
Antigen Retrieval: Tris-EDTA buffer, pH 8.0, 15 min

REFERENCE

There are currently no references for eIF4E antibody [HL1555] (GTX637030). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for eIF4E antibody [HL1555] (GTX637030). Be the first to share your experience with this product.
Package List Price ($)
$ 399
$ 169
Custom Conjugations