*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , 293T cytoplasm fraction extract , mouse colon
Rat, Rabbit, Bovine, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.52 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human eIF4E. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
eukaryotic translation initiation factor 4E,AUTS19,CBP,EIF4E1,EIF4EL1,EIF4F,eIF-4E
All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM]
GTX132092 WB Image
293T whole cell and cytoplasm extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody (GTX132092) diluted at 1:1000.
GTX132092 ICC/IF Image
eIF4E antibody detects eIF4E protein at cytoplasm by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: eIF4E protein stained by eIF4E antibody (GTX132092) diluted at 1:500.
Blue: Hoechst 33342 staining.
GTX132092 WB Image
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody (GTX132092) diluted at 1:1000.
GTX132092 IHC-P Image
eIF4E antibody detects EIF4E protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
eIF4E antibody (GTX132092) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min