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eIF4E antibody

Anti-eIF4E antibody used in Western Blot (WB). GTX132092
Anti-eIF4E antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132092
Anti-eIF4E antibody used in Western Blot (WB). GTX132092
Anti-eIF4E antibody used in IHC (Paraffin sections) (IHC-P). GTX132092

Cat No. GTX132092

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , 293T cytoplasm fraction extract , mouse colon

Predict Reactivity

Rat, Rabbit, Bovine, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human eIF4E. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

eukaryotic translation initiation factor 4E,AUTS19,CBP,EIF4E1,EIF4EL1,EIF4F,eIF-4E

Background

All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-eIF4E antibody used in Western Blot (WB). GTX132092

GTX132092 WB Image

293T whole cell and cytoplasm extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody (GTX132092) diluted at 1:1000.

Anti-eIF4E antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132092

GTX132092 ICC/IF Image

eIF4E antibody detects eIF4E protein at cytoplasm by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: eIF4E protein stained by eIF4E antibody (GTX132092) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-eIF4E antibody used in Western Blot (WB). GTX132092

GTX132092 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody (GTX132092) diluted at 1:1000.

Anti-eIF4E antibody used in IHC (Paraffin sections) (IHC-P). GTX132092

GTX132092 IHC-P Image

eIF4E antibody detects EIF4E protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
eIF4E antibody (GTX132092) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf