APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:100 |
| 1:50 - 1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EIF4E (NP_001959.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
eukaryotic translation initiation factor 4E , AUTS19 , CBP , EIF4E1 , EIF4EL1 , EIF4F , eIF-4E
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Database
Research Area
DATA IMAGES
|
GTX32580 IP Image
IP analysis of 293T cell lysate using GTX32580 eIF4E antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000
|
|
|
GTX32580 IHC-P Image
IHC-P analysis of human stomach tissue using GTX32580 eIF4E antibody.
Dilution : 1:100
|
|
|
GTX32580 IHC-P Image
IHC-P analysis of human lung cancer tissue using GTX32580 eIF4E antibody.
Dilution : 1:100
|
|
|
GTX32580 WB Image
WB analysis of various sample lysates using GTX32580 eIF4E antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane
|
|
|
GTX32580 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX32580 eIF4E antibody.
Blue : DAPI
Dilution : 1:100
|
REFERENCE
There are currently no references for eIF4E antibody (GTX32580). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for eIF4E antibody (GTX32580). Be the first to share your experience with this product.
