GeneTex
United States (US)

eIF4E antibody

GTX32580_20180530_IHC-P_1_18121410_777.jpg
GTX32580_20180530_WB_18121410_124.jpg
GTX32580_20180530_IHC-P_18121410_856.jpg

Cat No. GTX32580

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
See all eIF4E products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

25 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EIF4E (NP_001959.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Initiation Factor 4E,Auts19,Cbp,Eif4E1,Eif4El1,Eif4F,Eif-4E,Eif4E

Background

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES
GTX32580_20180530_IHC-P_1_18121410_777.jpg

GTX32580 IHC-P Image

IHC-P analysis of human stomach tissue using GTX32580 eIF4E antibody.
Dilution : 1:100
GTX32580_20180530_WB_18121410_124.jpg

GTX32580 WB Image

WB analysis of various samples using GTX32580 eIF4E antibody.The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane
GTX32580_20180530_IHC-P_18121410_856.jpg

GTX32580 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX32580 eIF4E antibody.
Dilution : 1:100
Package List Price ($)
$ 329