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eIF4E antibody

Anti-eIF4E antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32580
Anti-eIF4E antibody used in Western Blot (WB). GTX32580
Anti-eIF4E antibody used in IHC (Paraffin sections) (IHC-P). GTX32580
Anti-eIF4E antibody used in IHC (Paraffin sections) (IHC-P). GTX32580
Anti-eIF4E antibody used in Immunoprecipitation (IP). GTX32580

Cat. No. GTX32580

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all eIF4E products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:100
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EIF4E (NP_001959.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 4E , AUTS19 , CBP , EIF4E1 , EIF4EL1 , EIF4F , eIF-4E

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-eIF4E antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32580

GTX32580 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32580 eIF4E antibody.
Blue : DAPI
Dilution : 1:100

Anti-eIF4E antibody used in Western Blot (WB). GTX32580

GTX32580 WB Image

WB analysis of various sample lysates using GTX32580 eIF4E antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-eIF4E antibody used in IHC (Paraffin sections) (IHC-P). GTX32580

GTX32580 IHC-P Image

IHC-P analysis of human stomach tissue using GTX32580 eIF4E antibody.
Dilution : 1:100

Anti-eIF4E antibody used in IHC (Paraffin sections) (IHC-P). GTX32580

GTX32580 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX32580 eIF4E antibody.
Dilution : 1:100

Anti-eIF4E antibody used in Immunoprecipitation (IP). GTX32580

GTX32580 IP Image

IP analysis of 293T cell lysate using GTX32580 eIF4E antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399