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eIF4E antibody

Anti-eIF4E antibody used in Flow cytometry (FACS). GTX82525
Anti-eIF4E antibody used in Western Blot (WB). GTX82525
Anti-eIF4E antibody used in IHC (Paraffin sections) (IHC-P). GTX82525

Cat. No. GTX82525

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human, Squirrel
Package
400 μl ($399)
See all eIF4E products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50-1:100
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This EIF4E antibody is generated from rabbits immunized with a recombinant protein from human VGFR2.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

RRID

AB_11173866

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 4E , AUTS19 , CBP , EIF4E1 , EIF4EL1 , EIF4F , eIF-4E

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-eIF4E antibody used in Flow cytometry (FACS). GTX82525

GTX82525 FACS Image

FACS analysis of HL-60 cells using GTX82525 eIF4E antibody.
Top histogram : negative control
Bottom histogram : HL-60 cells

Anti-eIF4E antibody used in Western Blot (WB). GTX82525

GTX82525 WB Image

WB analysis of HL-60 cell lysate (35ug/lane) using GTX82525 eIF4E antibody.

Anti-eIF4E antibody used in IHC (Paraffin sections) (IHC-P). GTX82525

GTX82525 IHC-P Image

IHC-P analysis of human testis tissue using GTX82525 eIF4E antibody.

REFERENCE

Application Reference

Logan SM et al. Comp Biochem Physiol A Mol Integr Physiol 2019; 227 : 161-171 The squirrel with the lagging eIF2: Global suppression of protein synthesis during torpor.
Application : WB
Reactivity : Squirrel
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399