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eIF4E (phospho Ser209) antibody

Anti-eIF4E (phospho Ser209) antibody used in Western Blot (WB). GTX133606
Anti-eIF4E (phospho Ser209) antibody used in Western Blot (WB). GTX133606
Anti-eIF4E (phospho Ser209) antibody used in Western Blot (WB). GTX133606

Cat. No. GTX133606

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

Treated HCT116 , Treated HeLa , C2C12

Predict Reactivity

Rat, Rabbit, Bovine, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser209 of human eIF4E. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887046

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation initiation factor 4E , AUTS19 , CBP , EIF4E1 , EIF4EL1 , EIF4F , eIF-4E

Background

All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-eIF4E (phospho Ser209) antibody used in Western Blot (WB). GTX133606

GTX133606 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E (phospho Ser209) antibody (GTX133606) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-eIF4E (phospho Ser209) antibody used in Western Blot (WB). GTX133606

GTX133606 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with eIF4E (phospho Ser209) antibody (GTX133606) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-eIF4E (phospho Ser209) antibody used in Western Blot (WB). GTX133606

GTX133606 WB Image

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with eIF4E (phospho Ser209) antibody (GTX133606) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169