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eIF4E (phospho Ser209) antibody

Anti-eIF4E (phospho Ser209) antibody used in Western Blot (WB). GTX24774
Anti-eIF4E (phospho Ser209) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24774
Anti-eIF4E (phospho Ser209) antibody used in Western Blot (WB). GTX24774

Cat. No. GTX24774

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, FACS

Reactivity

Human, Mouse, Rat, Rabbit
Package
50 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:250
FACS 1:20
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human eIF4E that contains serine 209. The sequence is conserved in mouse, rat and rabbit.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation initiation factor 4E , AUTS19 , CBP , EIF4E1 , EIF4EL1 , EIF4F , eIF-4E

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-eIF4E (phospho Ser209) antibody used in Western Blot (WB). GTX24774

GTX24774 WB Image

WB analysis of whole cell extracts (20 μg lysate) of HeLa (lane 1), Serum Starved HeLa (lane 2), serum starved for overnight followed by Serum Released (lane 3), HEK-293 (lane 4), treated for 30 minutes with 25 μg/mL of Anisomycin (lane 5), L6 (lane 6) and L6 treated for 10 minutes with 200 ng/ml of Insulin (lane 7) using GTX24774 eIF4E (phospho Ser209) antibody.
Dilution : 1:500-1:2000

Anti-eIF4E (phospho Ser209) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24774

GTX24774 ICC/IF Image

ICC/IF analysis of U-87 MG cells using GTX24774 eIF4E (phospho Ser209) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

Anti-eIF4E (phospho Ser209) antibody used in Western Blot (WB). GTX24774

GTX24774 WB Image

WB (peptide competition) analysis of HeLa cells using GTX24774 eIF4E (phospho Ser209) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphoserine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399