WB (peptide competition) analysis of HeLa cells using GTX24774 eIF4E (phospho Ser209) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphoserine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.

WB analysis of whole cell extracts (20 μg lysate) of HeLa (lane 1), Serum Starved HeLa (lane 2), serum starved for overnight followed by Serum Released (lane 3), HEK-293 (lane 4), treated for 30 minutes with 25 μg/mL of Anisomycin (lane 5), L6 (lane 6) and L6 treated for 10 minutes with 200 ng/ml of Insulin (lane 7) using GTX24774 eIF4E (phospho Ser209) antibody.
Dilution : 1:500-1:2000

ICC/IF analysis of U-87 MG cells using GTX24774 eIF4E (phospho Ser209) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature