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eIF4G antibody

Anti-eIF4G antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50653
Anti-eIF4G antibody used in Western Blot (WB). GTX50653
Anti-eIF4G antibody used in IHC (Paraffin sections) (IHC-P). GTX50653

Cat. No. GTX50653

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all eIF4G products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

175 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.1229~1233 (P-V-S-P-L) derived from human eIF4G.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 4 gamma 1 , EIF-4G1 , EIF4F , EIF4G , EIF4GI , P220 , PARK18

Background

The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-eIF4G antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50653

GTX50653 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50653 eIF4G antibody.

Anti-eIF4G antibody used in Western Blot (WB). GTX50653

GTX50653 WB Image

WB analysis of extracts from HepG2 and HT-29 cells using GTX50653 eIF4G antibody.

Anti-eIF4G antibody used in IHC (Paraffin sections) (IHC-P). GTX50653

GTX50653 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX50653 eIF4G antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399