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eIF4G (phospho Ser1232) antibody

Anti-eIF4G (phospho Ser1232) antibody used in Western Blot (WB). GTX50344
Anti-eIF4G (phospho Ser1232) antibody used in IHC (Paraffin sections) (IHC-P). GTX50344

Cat No. GTX50344

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

175 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 1232 (P-V-S(p)-P-L) derived from human EIF4G.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation initiation factor 4 gamma 1 , EIF-4G1 , EIF4F , EIF4G , EIF4GI , P220 , PARK18

Background

The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-eIF4G (phospho Ser1232) antibody used in Western Blot (WB). GTX50344

GTX50344 WB Image

WB analysis of extracts from HT-29 cells untreated or treated with Anisomycin using GTX50344 EIF4G (phospho Ser1232) antibody.

Anti-eIF4G (phospho Ser1232) antibody used in IHC (Paraffin sections) (IHC-P). GTX50344

GTX50344 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX50344 EIF4G (phospho Ser1232) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

There are currently no reviews for eIF4G (phospho Ser1232) antibody (GTX50344). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319