GeneTex
United States (US)

eIF5A antibody, Internal (near N-term)

GTX88355_1001_IHC-P_18121411_198.jpg
GTX88355_1002_IHC-P_18121411_731.jpg
GTX88355_1003_IHC-P_18121411_588.jpg
GTX88355_2408_WB_18121411_326.jpg

Cat No. GTX88355

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88355-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-RPPHRASFLKRLESK, from the internal region (near N Terminus) of the protein sequence according to NP_001137232.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EIF5A1 Antibody , EIF5A Antibody , EIF5AI Antibody

Cellular Localization

Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.

Database

Research Area

DATA IMAGES
GTX88355_1001_IHC-P_18121411_198.jpg

GTX88355 IHC-P Image

IHC-P analysis of human spleen using GTX88355 eIF5A antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88355_1002_IHC-P_18121411_731.jpg

GTX88355 IHC-P Image

IHC-P analysis of human tonsil using GTX88355 eIF5A antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88355_1003_IHC-P_18121411_588.jpg

GTX88355 IHC-P Image

IHC-P analysis of human vessel using GTX88355 eIF5A antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88355_2408_WB_18121411_326.jpg

GTX88355 WB Image

WB analysis of Molt-4 cell lysate using GTX88355 eIF5A antibody, Internal (near N-term).
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer