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eNOS antibody, N-term

Anti-eNOS antibody, N-term used in Flow cytometry (FACS). GTX81622
Anti-eNOS antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81622
Anti-eNOS antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81622

Cat. No. GTX81622

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all eNOS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:10-1:50
IHC-P 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

133 kDa. ( Note )

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 48-75 amino acids from the N-terminal region of human NOS3.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nitric oxide synthase 3 , ECNOS , eNOS

Cellular Localization

Cell membrane,Cytoplasm, cytoskeleton,Golgi apparatus

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Database

Research Area

DATA IMAGES

Anti-eNOS antibody, N-term used in Flow cytometry (FACS). GTX81622

GTX81622 FACS Image

FACS analysis of HL-60 cells using GTX81622 eNOS antibody, N-term.
Green : primary antibody
Blue : negative control

Anti-eNOS antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81622

GTX81622 IHC-P Image

IHC-P analysis of human hepatocarcinoma using GTX81622 eNOS antibody, N-term.

Anti-eNOS antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81622

GTX81622 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX81622 eNOS antibody, N-term.
Green : eNOS
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399