*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
HUVEC , THP-1 , THP-1 (100nM PMA treatment for 6hr) , THP-1 (100nM PMA treatment for 24hr)
Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Guinea pig, Pig(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human eNOS. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
nitric oxide synthase 3 , ECNOS , eNOS
Cell membrane , Membrane , caveola , Cytoplasm , cytoskeleton , Golgi apparatus
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
GTX129113 WB Image
eNOS antibody detects eNOS protein by western blot analysis.
A. 30 μg THP-1 whole cell lysate/extract (untreated)
B. 30 μg THP-1 whole cell lysate/extract (100 nM Phorbol 12-Myristate 13-Acetate treatment for 6 hr)
C. 30 μg THP-1 whole cell lysate/extract (100 nM Phorbol 12-Myristate 13-Acetate treatment for 24 hr)
5 % SDS-PAGE
eNOS antibody (GTX129113) dilution: 1:1000
GTX129113 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with eNOS antibody (GTX129113) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.