GeneTex

  • Country / Location Selection

United States (US)

eNOS antibody

Orthogonal
Anti-eNOS antibody used in Western Blot (WB). GTX129113
Anti-eNOS antibody used in Western Blot (WB). GTX129113

Cat. No. GTX129113

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all eNOS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

133 kDa. ( Note )

Positive Control

THP-1 (100nM PMA treatment for 6hr) , THP-1 (100nM PMA treatment for 24hr) , HUVEC

Predict Reactivity

Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human eNOS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885898

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nitric oxide synthase 3 , ECNOS , eNOS

Cellular Localization

Cell membrane , Membrane , caveola , Cytoplasm , cytoskeleton , Golgi apparatus

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-eNOS antibody used in Western Blot (WB). GTX129113

GTX129113 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with eNOS antibody (GTX129113) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-eNOS antibody used in Western Blot (WB). GTX129113

GTX129113 WB Image

Orthogonal

eNOS antibody detects eNOS protein by western blot analysis.
A. 30 μg THP-1 whole cell lysate/extract (untreated)
B. 30 μg THP-1 whole cell lysate/extract (100 nM Phorbol 12-Myristate 13-Acetate treatment for 6 hr)
C. 30 μg THP-1 whole cell lysate/extract (100 nM Phorbol 12-Myristate 13-Acetate treatment for 24 hr)
5 % SDS-PAGE
eNOS antibody (GTX129113) dilution: 1:1000

REFERENCE

Application Reference

Jiang YH et al. J Urol 2016; Urothelial Barrier Deficits, Suburothelial Inflammation and Altered Sensory Protein Expressions in Detrusor Underactivity.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for eNOS antibody (GTX129113). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169