eNOS antibody

Cat No. GTX129843

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human

See all eNOS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent

Not tested in other applications.

Calculated MW

133 kDa. ( Note )

Positive Control

Jurkat , HUVEC , NCI-H929

Predict Reactivity

Mouse, Rat, Rabbit(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human eNOS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Nitric Oxide Synthase 3,Ecnos,Enos,Nos3

Cellular Localization

Cell membrane , Membrane , caveola , Cytoplasm , cytoskeleton , Golgi apparatus

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 4846
UniProt: P29474

Research Area

DATA IMAGES

GTX129843 WB Image

eNOS antibody detects eNOS protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with eNOS antibody (GTX129843) diluted by 1:2000.

GTX129843 WB Image

Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with eNOS antibody (GTX129843) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE