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eNOS antibody

Orthogonal
Anti-eNOS antibody used in Western Blot (WB). GTX129843
Anti-eNOS antibody used in Western Blot (WB). GTX129843

Cat. No. GTX129843

Reference 2
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all eNOS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

133 kDa. ( Note )

Positive Control

Jurkat , NCI-H929 , HUVEC

Predict Reactivity

Mouse, Rabbit, Sheep, Bovine, Dog, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human eNOS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886105

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nitric oxide synthase 3 , ECNOS , eNOS

Cellular Localization

Cell membrane , Membrane , caveola , Cytoplasm , cytoskeleton , Golgi apparatus

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-eNOS antibody used in Western Blot (WB). GTX129843

GTX129843 WB Image

eNOS antibody detects eNOS protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with eNOS antibody (GTX129843) diluted by 1:2000.

Anti-eNOS antibody used in Western Blot (WB). GTX129843

GTX129843 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with eNOS antibody (GTX129843) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Garc?a-Arroyo FE et al. Biomolecules 2019; 9 (10) Allopurinol Prevents the Lipogenic Response Induced by an Acute Oral Fructose Challenge in Short-Term Fructose Fed Rats.
Linghu K et al. Eur J Pharmacol 2016; 789 : 195-201 Ameliorating effects of 1,8-cineole on LPS-induced human umbilical vein endothelial cell injury by suppressing NF-κB signaling in vitro.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

eNOS antibody Cat. No. GTX129843

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-eNOS antibody used in Western Blot (WB). GTX129843
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Biomolecules in 2019.PMID: 31614639
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169