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eNOS (phospho Ser1177) antibody [BDI250]

Anti-eNOS (phospho Ser1177) antibody [BDI250] used in Western Blot (WB). GTX42866

Cat. No. GTX42866

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 BDI250

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Human, Mouse
Package
50 μg ($399)
See all eNOS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/ml
ELISA 0.1 μg/ml
Not tested in other applications.

Calculated MW

133 kDa. ( Note )

Product Note

Endothelial NO synthase produces nitric oxide (NO) which plays a crucial role in cardiovascularhomeostasis. Activation of the enzyme and NO production can be induced by phosphorylation of eNOS atSerine 1177 by activated Akt/PKB. Specifically recognizes eNOS phosphorylated at serine 1177.MW = 140 kDa. Cross-reacts with human and mouse.

PROPERTIES

Form

Liquid

Buffer

PBS, PEG, Sucrose, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.10 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Synthetic phosphopeptide corresponding to amino acid residues surrounding S1177.

Purification

Purified by thiophilic adsorption and size exclusion chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nitric oxide synthase 3 , ECNOS , eNOS

Cellular Localization

Cell membrane , Cytoplasm, cytoskeleton , Golgi apparatus

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Database

Research Area

DATA IMAGES

Anti-eNOS (phospho Ser1177) antibody [BDI250] used in Western Blot (WB). GTX42866

GTX42866 WB Image

WB analysis of Bend3 cells using GTX42866 eNOS (phospho Ser1177) antibody [BDI250] at 1 μg/mL.
Lane 1 : Untreated whole cell lysate
Lane 2 : Pervanadate-treated whole cell lysate

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399